| Project/Area Number |
16K14725
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kobe University |
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Principal Investigator |
Tsujita Kazuya 神戸大学, バイオシグナル総合研究センター, 講師 (10457054)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 細胞膜の張力 / BARタンパク質 / BARドメイン / 膜変形タンパク質 |
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| Outline of Final Research Achievements |
Tension in the plasma membrane plays an important roles in mechanical cellular functions, such as cell migration and division. however, it is unclear how plasma membrane tension regulates these processes in space and time. In this study, we identified membrane bending BAR domain of FBP17 as the probe for plasma membrane tension. Using the GFP-fusion probe combined with live imaging, we found that this probe dynamically localized to the plasma membrane by sensing the fluctuation of membrane tension during cell migration. Thus, this probe allows us to visualize a gradient of membrane tension in mechanical cellular processes.
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