| Project/Area Number |
16K14731
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Rikkyo University |
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Principal Investigator |
GOTO Satoshi 立教大学, 理学部, 教授 (60280575)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Miki 立教大学, 理学部, 助教 (40301783)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | GPI / 核膜 / 小胞体 / 品質管理 / ショウジョウバエ / PIGB |
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| Outline of Final Research Achievements |
Glycophosphatidylinositol (GPI), a glycolipid synyhesized in the endoplasmic reticulum (ER) is a key molecule to anchor proteins to the plasma membrane. We previously found that one of the GPI synthesis enzymes PigB is localized to the nuclear envelope (ER) whereas the other enzymes are in the ER. In this study, we examined whether the ER localization of PigB is required for its function. We first generated ER-localizing PigB (ER type) that has normal activity. The PigB (ER type) did not resuce lethality of pigB mutation whereas PigB (NE type)
fully rescued it. This result shows that the ER localization of PigB is essential for its function.
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