| Project/Area Number |
16K14754
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
町田 千代子 中部大学, 応用生物学部, 教授 (70314060)
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| Research Collaborator |
Suzuki Takanori
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 植物ウイルス / 病徴タンパク質 / 病徴の伝搬 / 葉の発生・分化 |
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| Outline of Final Research Achievements |
Pathogenic protein βC1 of tomato yellow leaf curl virus binds to AS1 in Arabidopsis thaliana and enhances the symptoms of the virus by inducing RNA silencing in the plant. When βC1 is transiently expressed in the lower leaves of tobacco plants, symptoms appear in the upper leaves, not in the infected leaves. Therefore, it is possible that βC1-related molecules (proteins and RNAs) might be transported long distances in the plants. We here have shown that tobacco AS1 is also necessary for the appearance of symptoms in the upper leaves due to βC1. In addition, grafting experiments were conducted to investigate whether AS1 was necessary for migration or for the appearance of symptoms in the upper lobes thereafter. However, although the importance of AS1 was understood, the answer was not obtained because the graft itself is inhibitory to the appearance of the symptom.
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| Academic Significance and Societal Importance of the Research Achievements |
AS1のC末端側領域がβC1に結合することを明らかにし、さらにその領域のペプチドを、βC1をトラップするデコイとして利用でき、ウイルスの感染に強い植物を作成する方途が開けた。
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