Phenotypic variation in bacterial communication

• Project/Area Number: 16K14795
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: University of Tsukuba
• Principal Investigator: TOYOFUKU Masanori 筑波大学, 生命環境系, 准教授 (30644827)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 微生物間コミュニケーション / 相変異 / 細菌間コミュニケーション / 細菌間相互作用 / 挿入因子 / 変異 / 細菌間情報伝達 / 環境応答 / 環境適応 / シグナル伝達 / バイオフィルム

Outline of Final Research Achievements

Communication among individuals is vital in most of the living systems including bacteria. In order to gain insight into the elusion of bacterial communication we analyzed several environmental isolates of Pseudmomas aeruginosa and compared their quorum sensing systems. We found that the quorum sensing system varies among the isolates and ones isolated from poor nutrient environments often had mutations in the quorum sensing systems. Interestingly some of the strains carried insertion sequences in the quorum sensing genes, which could jump in and out from the gene. This allowed the strain to switch on and off the quorum sensing system depending on the environment. In summary we found a unique phase variation system in bacterial communication where it is regulated by insertion sequence.

Academic Significance and Societal Importance of the Research Achievements

微生物間コミュニケーション研究のほとんどが病原性に関連したものであり，生態学的な研究は少ない。特に，系統地理学的な研究は皆無である。また、従来のコミュニケーション研究はいわゆる高等な生物を対象に行われるものがほとんどであり、,遺伝子レベルでの系統学的解析は困難であった。単純な細菌のコミュニケーションだからこそ，コミュニケーションと環境・生態の関係に遺伝子レベルで迫ることができた。

Research Products

• [Int'l Joint Research] チューリッヒ大学(スイス)
• [Int'l Joint Research] チューリッヒ大学(スイス)
• [Int'l Joint Research] チューリッヒ大学/ETH(スイス)
• [Int'l Joint Research] HelmholtzZentrum(ドイツ)
• [Journal Article] Cracking open bacterial membrane vesicles (2020)
  • Author(s): Nagakubo T, Nomura N, Toyofuku M.
  • Journal Title: Frontiers in Microbiology Volume: 10 Pages: 3026-3026
  • DOI: 10.3389/fmicb.2019.03026
  • NAID: 120007132782
  • Peer Reviewed / Open Access
• [Journal Article] Bacterial communication through membrane vesicles (2019)
  • Author(s): Toyofuku M
  • Journal Title: Biotechnol Biochem Volume: 83 Issue: 9 Pages: 1599-1605
  • DOI: 10.1080/09168451.2019.1608809
  • Peer Reviewed
• [Journal Article] Types and origins of bacterial membrane vesicles (2019)
  • Author(s): Toyofuku M, Nomura N, Eberl L
  • Journal Title: Nature Reviews Microbiology Volume: 17 Issue: 1 Pages: 13-24
  • DOI: 10.1038/s41579-018-0112-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Paracoccus denitrificans can utilize various long-chain N-acylhomoserine lactones and sequester them in membrane vesicles (2018)
  • Author(s): Morinaga K, Yamamoto T, Nomura N, Toyofuku M
  • Journal Title: Environmental Microbiology Reports Volume: 10 Issue: 6 Pages: 651-654
  • DOI: 10.1111/1758-2229.12674
  • Peer Reviewed / Open Access
• [Journal Article] Nitric oxide, an old molecule with noble functions in Pseudomonas aeruginosa biology (2018)
  • Author(s): Toyofuku M, Yoon SS
  • Journal Title: Advances in Microbial Physiology Volume: 72 Pages: 117-145
  • DOI: 10.1016/bs.ampbs.2018.01.005
  • ISBN: 9780128144138
• [Journal Article] 細菌が放出する膜小胞の新たな展開 (2018)
  • Author(s): 豊福雅典
  • Journal Title: 化学と生物 Volume: 56 Pages: 79-80
• [Journal Article] Prophage-triggered membrane vesicle formation through peptidoglycan damage in Bacillus subtilis (2017)
  • Author(s): M. Toyofuku, G. Carcamo-Oyarce, T. Yamamoto, F. Eisenstein, C. Hsiao, M. Kurosawa, K. Gademann, M. Pilhofer, N. Nomura, and L. Eberl
  • Journal Title: Nature communications Volume: 8 Issue: 1
  • DOI: 10.1038/s41467-017-00492-w
  • NAID: 120007134777
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] What will membrane vesicles (MVs) bring to bacterial communication? (2017)
  • Author(s): Masanori Toyofuku, Nobuhiko Nomura
  • Journal Title: Microbes and Environments Volume: 32 Issue: 3 Pages: 185-187
  • DOI: 10.1264/jsme2.ME3203rh
  • NAID: 130006105324
  • ISSN: 1342-6311, 1347-4405
  • Open Access
• [Journal Article] A Versatile and Rapidly Deployable Device to Enable Spatiotemporal Observations of the Sessile Microbes and Environmental Surfaces (2017)
  • Author(s): Kiyokawa T, Usuba R, Obana N, Yokokawa M, Toyofuku M, Suzuki H, Nomura N
  • Journal Title: Microbes and Environments Volume: 32 Issue: 1 Pages: 88-91
  • DOI: 10.1264/jsme2.ME16161
  • NAID: 130005519591
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Membrane vesicle-mediated bacterial communication (2017)
  • Author(s): Toyofuku M, Morinaga K, Hashimoto Y, Uhl J, Shimamura H, Inaba H, Schmitt-Kopplin P, Eberl L, Nomura N
  • Journal Title: ISME J. Volume: 印刷中 Issue: 6 Pages: 1504-1509
  • DOI: 10.1038/ismej.2017.13
  • NAID: 120007129099
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Biofilm formation by Paracoccus denitrificans requires a type I secretion system dependent adhesin BapA (2017)
  • Author(s): Yoshida K, Toyofuku M, Obana N, Nomura N
  • Journal Title: FEMS Microbiol Lett. Volume: 364 Issue: 4
  • DOI: 10.1093/femsle/fnx029
  • Peer Reviewed
• [Journal Article] Influence of the alginate production on cell-to-cell communication in Pseudomonas aeruginosa PAO1 (2017)
  • Author(s): Yang J, Toyofuku M, Sakai R, Nomura N
  • Journal Title: Environ Microbiol Rep. Volume: 印刷中 Issue: 3 Pages: 239-249
  • DOI: 10.1111/1758-2229.12521
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] メゾスコピック微生物学の夜明け (2016)
  • Author(s): 豊福雅典，森永花菜, 野村暢彦
  • Journal Title: 環境バイオテクノロジー学会誌 Volume: 16 Pages: 23-29
• [Journal Article] 細胞破裂を介した細菌のバイオフィルムとベシクル形成 (2016)
  • Author(s): 豊福雅典，野村暢彦
  • Journal Title: バイオサイエンスとインダストリー Volume: 74 Pages: 420-421
• [Journal Article] 微生物が能動的に産生するメンブランベシクルの生合成と機能 (2016)
  • Author(s): 尾花望, 黒沢正治，豊福雅典, 野村暢彦
  • Journal Title: 化学と生物 Volume: 54 Pages: 812-819
  • NAID: 130006176422
• [Presentation] 可動性因子を用いた緑膿菌の細胞間コミュニケーションの切替 (2019)
  • Author(s): 須澤 由希, 遠矢 正城, 尾花 望, 吉澤 晋, 木暮 一啓, 野村 暢彦, 豊福 雅典
  • Organizer: 第33回 日本微生物生体学会
• [Presentation] ベシクルを介した細菌の化学コミュニケーション (2019)
  • Author(s): 豊福雅典
  • Organizer: 第１４回化学生態研究会
  • Invited
• [Presentation] How do bacteria “really” communicate? (2019)
  • Author(s): Masanori Toyofuku
  • Organizer: Marine Biotechnology Conference 2019
  • Int'l Joint Research / Invited
• [Presentation] 膜小胞を介した細菌間の情報伝達 (2019)
  • Author(s): 豊福雅典
  • Organizer: 化学コミュニケーションのフロンティア第２回若手シンポジウム
  • Invited
• [Presentation] バクテリアが放出する膜小胞の利用に向けて (2019)
  • Author(s): 豊福雅典
  • Organizer: 放線菌学会学術講演会
  • Invited
• [Presentation] メンブレンベシクルからみえてきた細菌の多様な情報戦略 (2018)
  • Author(s): 豊福雅典
  • Organizer: 日本微生物生態学会
  • Invited
• [Presentation] Challenging the dogma of bacterial membrane vesicle formation (2018)
  • Author(s): 豊福雅典
  • Organizer: 10th Asian Symposium on Microbial Ecology
  • Int'l Joint Research / Invited
• [Presentation] 細菌のメンブレンベシクル形成機構にまつわるドグマへの挑戦 (2018)
  • Author(s): 豊福雅典
  • Organizer: 第２８回植物細菌病談話会
  • Invited
• [Presentation] 低分子化合物及び膜小胞を介した細菌間相互作用に関する研究 (2018)
  • Author(s): 豊福雅典
  • Organizer: 日本農芸化学会 2018年度第３回関東支部例会
  • Invited
• [Presentation] メンブレンベシクルを介した細菌間コミュニケーション (2018)
  • Author(s): 豊福雅典
  • Organizer: 日本細菌学会総会
• [Presentation] 低分子化合物及び膜小胞を介した細菌間相互作用に関する研究 (2018)
  • Author(s): 豊福雅典
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] 細菌によるデジタルな情報伝達!? (2017)
  • Author(s): 豊福雅典，森永花菜，Leo Eberl ,野村暢彦
  • Organizer: 日本生物工学会
• [Presentation] Membrane vesicle formation through cell death (2017)
  • Author(s): Masanori Toyofuku, Nobuhiko Nomura, Leo Eberl
  • Organizer: FEMS Microbiology Congress 2017
• [Book] Bacterial Membrane Vesicles (2020)
  • Author(s): Toyofuku M, Tashiro Y, Nomura N, Eberl L
  • Total Pages: 18
  • Publisher: Springer
• [Book] 現代コロイド界面化学の基礎 (2018)
  • Author(s): 豊福雅典，野村暢彦
  • Total Pages: 2
  • Publisher: 丸善出版株式会社
  • ISBN: 4621302914
• [Book] 食と微生物の辞典 (2017)
  • Author(s): 豊福雅典 他199名
  • Total Pages: 512
  • Publisher: 朝倉書店
  • ISBN: 9784254431216
• [Book] “Bacterial interactions” In: Encyclopedia of Biocolloid and Biointerface Sciences (2016)
  • Author(s): Masanori Toyofuku, Youske Tashiro, Inaba Tomohiro, Nobuhiko Nomura
  • Publisher: Wiley
• [Remarks] 研究室HP
  • URL: http://www.envr.tsukuba.ac.jp/~microbio/
• [Remarks] 筑波大学 MiCS
  • URL: https://www.mics.tsukuba.ac.jp
• [Remarks] 研究室HP
  • URL: http://www.envr.tsukuba.ac.jp/~microbio/index.html