| Project/Area Number |
16K14813
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ecology/Environment
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| Research Institution | Fukui University of Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
谷藤 吾朗 独立行政法人国立科学博物館, 動物研究部, 研究員 (70438480)
中澤 昌美 大阪府立大学, 生命環境科学研究科, 助教 (90343417)
矢吹 彬憲 国立研究開発法人海洋研究開発機構, 海洋生物多様性研究分野, 研究員 (20711104)
横山 亜紀子 国立研究開発法人国立環境研究所, 地域環境研究センター, 特別研究員 (30466601)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | chlorophylls / chlorophyll catabolism / algivorous protists / クロロフィル / クロロフィルの分解代謝 / 藻類食プロティスト / 微細藻類 / プロティスト / クロロフィル分解 / 微生物間相互作用 / CPE代謝（シクロエノール代謝） / ファゴサイトーシス / 進化 |
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| Outline of Final Research Achievements |
We elucidated that CPE catabolism is ubiquitous chlorophyll degradation process in eukaryotes from extensive experiments and pigment analyses on two-membered cultures protistan predators and algal prey as well as unialgal cultures of microalgae; thus, the results showed that CPE catabolism is also common among many of algal lineages with the secondary plastids. We then carried out a comparative transcriptome analysis on a protistan predator that feeds on both cyanobacteria (performing CPE catabolism) and non-phototrophic bacteria and inferred changes in a gene expression profile responding upon predation of chlorophyll-containing cyanobacteria. Furthermore, we suggested by a reverse genetic approach, the RNAi knockdown experiments on Euglena gracilis, that a part of genes responsible for degradation of chlorophylls known among green plants are preserved and functioned in the secondary chloroplasts of euglenophytes algae.
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