Identification and characterization of a novel non-S-specific factor required for self-incompatibility in Solanaceae
| Project/Area Number |
16K14826
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Chiba University |
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Principal Investigator |
SASSA Hidenori 千葉大学, 大学院園芸学研究科, 教授 (50295507)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 自家不和合性 / ナス科 / 非S因子 / S遺伝子座 / S-RNase / 雌ずい / 花粉 / ペチュニア |
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| Outline of Final Research Achievements |
Self/non-self recognition in self-incompatible plants is generally controlled by single multiallelic S locus. S locus genes have extensively been studied, however, knowledge on non-S-specific factors is limited. This study aimed to identify and characterize novel non-S-specific factor in a solanaceous plant Petunia. A good candidate for non-S-specific factor, 2H12, was identified. RNAi-mediated knock-down of 2H12 resulted in breakdown of self-incompatibility, suggesting that 2H12 is a novel non-S-specific factor.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では新規の非S因子として2H12を発見した。今後2H12の機能を詳細に解析することで、自家不和合性機構の理解に寄与する重要な知見が得られると期待される。
ナス科植物であるじゃがいもは遺伝的改良が進んでおらず、純系親同士の交配による一代雑種品種の作出が望まれているが、そのためには自家不和合性の打破が望まれる。しかしS遺伝子のゲノム編集による破壊で自家不和合性打破は可能だが、S遺伝子は非常に多様性が高く、ゲノム編集の標的には適さない。一方で2H12は保存的なので、ゲノム編集でじゃがいもの2H12を破壊することで自家和合性じゃがいもの育成が可能になり、品種改良に寄与できる可能性がある。
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Report
(5 results)
Research Products
(1 results)
