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Identification and characterization of a novel non-S-specific factor required for self-incompatibility in Solanaceae

Research Project

Project/Area Number 16K14826
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Science in genetics and breeding
Research InstitutionChiba University

Principal Investigator

SASSA Hidenori  千葉大学, 大学院園芸学研究科, 教授 (50295507)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords自家不和合性 / ナス科 / 非S因子 / S遺伝子座 / S-RNase / 雌ずい / 花粉 / ペチュニア
Outline of Final Research Achievements

Self/non-self recognition in self-incompatible plants is generally controlled by single multiallelic S locus. S locus genes have extensively been studied, however, knowledge on non-S-specific factors is limited. This study aimed to identify and characterize novel non-S-specific factor in a solanaceous plant Petunia. A good candidate for non-S-specific factor, 2H12, was identified. RNAi-mediated knock-down of 2H12 resulted in breakdown of self-incompatibility, suggesting that 2H12 is a novel non-S-specific factor.

Academic Significance and Societal Importance of the Research Achievements

本研究では新規の非S因子として2H12を発見した。今後2H12の機能を詳細に解析することで、自家不和合性機構の理解に寄与する重要な知見が得られると期待される。
ナス科植物であるじゃがいもは遺伝的改良が進んでおらず、純系親同士の交配による一代雑種品種の作出が望まれているが、そのためには自家不和合性の打破が望まれる。しかしS遺伝子のゲノム編集による破壊で自家不和合性打破は可能だが、S遺伝子は非常に多様性が高く、ゲノム編集の標的には適さない。一方で2H12は保存的なので、ゲノム編集でじゃがいもの2H12を破壊することで自家和合性じゃがいもの育成が可能になり、品種改良に寄与できる可能性がある。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (1 results)

All 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results)

  • [Journal Article] High Genomic Affinity between <i>Torenia baillonii</i> and <i>Torenia fournieri</i> Revealed by Genome Analysis Using a Triploid Hybrid2017

    • Author(s)
      (1)Nuntha, B., Kikuchi, S., Taychasinpitak, T., Sassa, H. and Koba, T.
    • Journal Title

      CYTOLOGIA

      Volume: 82 Issue: 2 Pages: 213-218

    • DOI

      10.1508/cytologia.82.213

    • NAID

      130006769799

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

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