| Project/Area Number |
16K14828
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Tsutsumi Nobuhiro 東京大学, 大学院農学生命科学研究科(農学部), 教授 (00202185)
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| Co-Investigator(Kenkyū-buntansha) |
西村 明日香 東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (70767342)
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| Project Period (FY) |
2016-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | プラスチド / 形質転換 / オルガネラ / 選抜マーカー / プラスチドゲノム / 亜硝酸選抜 / パーティクルガン |
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| Outline of Final Research Achievements |
For the purpose of developing rice plastid transformation method, we improved and examined cultivation methods and transgene selection systems. First, in order to improve gene transfer efficiency, a culture system of plastid developed green tissue was examined, and the shoot apex with developing leaves and a multiple shoot induction culture system of the seedling were developed. In addition, plastid transformation by the newly developed nitrite selection method using the conventional rice callus was carried out and it succeeded in highly efficient gene introduction. It was also confirmed that the transgene was retained in the next generation (T1). Through the detailed analysis of transformed plants, we will further study the improvement to establish the plastid transformation method in rice.
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