| Project/Area Number |
16K14859
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Yokohama National University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 担子菌 / さび病菌 / 遺伝子解析 / ゲノム / 乾燥標本 / ゲノム解析 / NGS / ２本鎖RNA / 植物寄生菌 |
|---|
| Outline of Final Research Achievements |
Although the importance as plant pathogens application of molecular genetics in rust fungus research has been rather limited because of the difficulties in axenic culture. On the other hand, numerous number of historical specimens of rust fungi are readily available as dried samples and the use of the dried specimens as resources of genetic information is a promising approach toward the genetic studies of rust fungus species. In this project, I selected Stereostratum corticioides (bamboo culm rust) because of its availability of the samples. To prepare DNA and RNA samples suitable for the mass-analysis of next-generation sequencers, I investigated the protocols and conditions for extraction methods to obtain long double-stranded DNA samples from teliospores of S. corticioides.
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