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Detection and identification of complement components in bony fish skin mucus and their interactome analysis

Research Project

Project/Area Number 16K14985
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionKyushu University

Principal Investigator

NAKAO Miki  九州大学, 農学研究院, 教授 (50212080)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords免疫 / 生体防御 / 補体 / 体表 / 粘液 / インタラクトーム / 相互作用 / 魚類 / インタラクトー無 / インタラクト―ム
Outline of Final Research Achievements

The complement system is a major humoral factor in innate immunity, and in mammals its components are mainly synthesized in the liver and are thought to function in body fluids such as plasma. In fish, gene expression of complement components has been confirmed in organs and regions other than liver, but there are many unknown points about the distribution in the fish of the complement component protein and the function in the area. In this study, we focused on body surface mucus, which is the first barrier in the body defense, and aimed to identify the complement components present in mucus and clarify the reaction mechanism. As the first step, it was attempted to clarify the presence of complement components in carp surface mucus at the protein level by Western blot using antibodies against each complement component of carp.

Academic Significance and Societal Importance of the Research Achievements

本研究では、魚類の体表、生殖腺、脳などで発現することが判明している補体成分の、それら局所での未知の機能を明らかにするために、局所で補体成分が相互作用する分子を網羅的に探索した。このアプローチは、既知の補体系内での機能という先入観にとらわれず、補体成分の新機能に推定することができる。本研究の考え方と方法論は、補体成分のみならず、ゲノムデータ中で配列と発現部位しかわかっていない多くの機能未知遺伝子について、それらの生物学的役割りに迫る一般的な研究戦略として展開可能となる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (25 results)

All 2019 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (20 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Generation of virus-specific CD8+ T cells by vaccination with inactivated virus in the intestine of ginbuna crucian carp.2019

    • Author(s)
      Tajimi S, Kondo M, Nakanishi T, Nagasawa T, Nakao M, Somamoto T.
    • Journal Title

      Developmental and Comparative Immunology

      Volume: 93 Pages: 37-44

    • DOI

      10.1016/j.dci.2018.12.009

    • NAID

      120006819494

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of Anti-atypical <i>Aeromonas salmonicida</i> Monoclonal Antibodies for Diagnosis of &ldquo;New Ulcer Disease&rdquo; in Koi Carp2018

    • Author(s)
      Tomonori Somamoto, Shinpei Maruyama, Takahiro Nagasawa, Miki Nakao, Atsushi Sato, Hajime Hatta, Mitsuru Sato, Yukie Murakami-Yamaguchi, Kumiko Kizu-Mori, Yuki Hirakawa, Hiroshi Narita
    • Journal Title

      Fish Pathology

      Volume: 53 Issue: 1 Pages: 36-39

    • DOI

      10.3147/jsfp.53.36

    • NAID

      130006619196

    • ISSN
      0388-788X, 1881-7335
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive validation of T- and B-cell deficiency in rag1-null zebrafish: Implication for the robust innate defense mechanisms of teleosts.2017

    • Author(s)
      Tokunaga Y, Shirouzu M, Sugahara R, Yoshiura Y, Kiryu I, Ototake M, Nagasawa T, Somamoto T, Nakao M.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 7536-7536

    • DOI

      10.1038/s41598-017-08000-2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of probiotic Bacillus aerius B81e isolated from healthy hybrid catfish on growth, disease resistance and innate immunity of Pla-mong Pangasius bocourti.2017

    • Author(s)
      Meidong R, Khotchanalekha K, Doolgindachbaporn S, Nagasawa T, Nakao M, Sakai K, Tongpim S.
    • Journal Title

      Fish Shellfish Immunol.

      Volume: 73 Pages: 1-10

    • DOI

      10.1016/j.fsi.2017.11.032

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 繊毛虫Ichtyophthirius multifiliisの成長段階の違いによるギンブナの免疫応答の比較2019

    • Author(s)
      塩田昂明, 助田将樹, 中西照幸, 長澤 貴宏, 杣本智軌, 中尾実樹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 魚類炎症反応におけるC5aの役割解明のための抗ゼブラフィッシュC5a抗体の作成2019

    • Author(s)
      斎藤武尊, 長澤 貴宏, 杣本智軌, 中尾実樹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 特異抗体を利用したコイ補体成分C5の迅速精製2019

    • Author(s)
      中尾実樹, 木村美智代, 長澤 貴宏, 杣本智軌
    • Organizer
      日本水産学会春季大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] コイ体表粘液中に存在する補体成分の検出2018

    • Author(s)
      吉迫郁子, 黒木将武, 長澤貴宏, 杣本智軌, 中尾実樹
    • Organizer
      日本比較免疫学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Phylogenetic inference on functions of the classical complement pathway in bony fish2018

    • Author(s)
      Nakao M, Iwanaga S, Nagasawa T, Somamoto T
    • Organizer
      14th Congress of International Society of Developmental and Comparative Immunology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Viral ligands recognition of carp thrombocytes as natural type I interferon producing cells2018

    • Author(s)
      Nagasawa T, Somamoto T, Nakao M
    • Organizer
      14th Congress of International Society of Developmental and Comparative Immunology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evolutionary implication on the function of the classical pathway of the complement system2018

    • Author(s)
      Nakao M, Iwanaga S, Nagasawa T, Somamoto T
    • Organizer
      Asian Invertebrate Immunity Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Type I IFN production by carp thrombocytes as professional antiviral leukocytes, like mammalian plasmacytoid dendritic cells, via IRF7 signaling pathway2018

    • Author(s)
      Nagasawa T, Somamoto T, Nakao M
    • Organizer
      Asian Invertebrate Immunity Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 硬骨魚類における古典経路の機能解析からの系統発生学的考察2018

    • Author(s)
      中尾実樹, 岩永彩代、長沢貴宏、杣本智軌
    • Organizer
      日本補体学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ギンブナCD8+Tリンパ球の細胞外寄生原虫に対する細胞障害機構の解明2018

    • Author(s)
      助田将樹、長沢貴宏、中尾実樹、杣本智軌
    • Organizer
      日本水産学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Homeostatic functional analysis of Tecrem, a CD46-like complement regulatory protein, on epithelial cells in carp fish2018

    • Author(s)
      Prakash H, Motobe S, Nagasawa T, Somamoto T, Nakao M
    • Organizer
      日本水産学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] コイ補体 C7 アイソタイプの機能解析2017

    • Author(s)
      野口真代・長沢貴宏・杣本智軌・中尾実樹
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2017-03-26
    • Related Report
      2016 Research-status Report
  • [Presentation] コイ補体成分B/C2-Bの組換え体作成と機能解析2017

    • Author(s)
      赤司小百合、長沢貴宏、杣本智軌、中尾実樹
    • Organizer
      日本比較免疫学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] コイ栓球のI型インターフェロン産生能2017

    • Author(s)
      長沢貴宏、杣本智軌、中尾実樹
    • Organizer
      日本比較免疫学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] コイ補体Properdinアイソタイプの機能解析2017

    • Author(s)
      黒木将武、吉岡和紀、長沢貴宏、杣本智軌、中尾実樹
    • Organizer
      日本補体学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] コイ補体C7アイソタイプの機能解析2017

    • Author(s)
      野口真代、長沢貴宏、杣本智軌、中尾実樹
    • Organizer
      日本補体学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Functional diversity of two C7 isotypes in bony fish, a primitive vertebrate model2017

    • Author(s)
      Nakao M, Noguchi M, Akahoshi S, Nagasawa T, Somamoto T
    • Organizer
      European Meeting on Complement in Human Disease
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of tecrem, a CD46-like complement regulatory protein, on epithelial cells in the common carp2017

    • Author(s)
      Prakash H, Motobe S, Nagasawa T, Somamoto T, Nakao M
    • Organizer
      The JSFS 85th Anniversary-Commemorative International Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] アフィニティーシルクの魚病細菌感染症診断への応用2017

    • Author(s)
      丸山真平、佐藤充、小島桂、佐藤巧視、長沢貴宏、中尾実樹、杣本智軌
    • Organizer
      日本魚病学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 繊毛虫Icthyophthirius multifiliisに対する魚類CD8+T細胞の傷害機構2017

    • Author(s)
      助田将樹、長沢貴宏、中尾実樹、杣本智軌
    • Organizer
      日本魚病学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Book] 動物学の百科事典、pp 508-509「補体系の進化」2018

    • Author(s)
      公益社団法人日本動物学会(中尾実樹)
    • Total Pages
      800
    • Publisher
      丸善出版
    • ISBN
      9784621303092
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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