Serotonin improves high fat diet induced obesity in mice by the activations of PGC-1 alpha and UCP1 through 5-HTR2A and 7

• Project/Area Number: 16K15021
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Tohoku University
• Principal Investigator: Aso Hisashi 東北大学, 農学研究科, 教授 (50241625)
• Co-Investigator(Kenkyū-buntansha): 北澤 春樹 東北大学, 農学研究科, 准教授 (10204885) ; 野地 智法 東北大学, 農学研究科, 准教授 (10708001) ; 渡邊 康一 東北大学, 農学研究科, 助教 (80261494)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: セロトニン / 褐色脂肪細胞 / THP1 / エネルギー代謝 / 抗肥満作用 / 抗肥満効果

Outline of Final Research Achievements

Mice were fed a chow (Ch) diet or a high fat (F) diet and orally administrated with serotonin (5-HT) every morning between the ages of 5 and 26 weeks. Mice on the F diet gained significantly more weight than the Ch fed mice. In order to identify the target tissues of the effect of 5-HT on energy metabolism, we focused on brawn adipose tissue. PGC-1 is a master regulator that promotes mitochondrial biogenesis and an activity of uncoupling protein (UCP) 1 in brawn adipose tissue. 5-HT increased the expressions of PGC-1-a, b, c and UCP1. Brawn adipose tissue had higher expressions of 5HTR2A, 2B and 7. Mice were pre-treated with several 5-HTR antagonists at 30 min before 5-HT injection. We revealed that 5-HT increased the mRNA expressions of PGC-1and UCP1 through 5-HTR2A and 7.

Research Products

• [Journal Article] Immunobiotics for the bovine host: their interaction with intestinal epithelial cells and their effect on antiviral immunity. (2018)
  • Author(s): Julio Villena, Hisashi Aso, Victor P. Rutten, Hideki Takahashi, Willem Van Eden and Haruki Kitazawa
  • Journal Title: Frontiers in Immunology Volume: 9 Pages: 326-326
  • DOI: 10.3389/fimmu.2018.00326
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of a novel mechanism of action of bovine IgG antibodies specific for Staphylococcus aureus (2018)
  • Author(s): Furukawa Mutsumi、Yoneyama Hiroshi、Hata Eiji、Iwano Hidetomo、Higuchi Hidetoshi、Ando Tasuke、Sato Mika、Hayashi Tomohito、Kiku Yoshio、Nagasawa Yuya、Niimi Kanae、Usami Katsuki、Ito Kumiko、Watanabe Kouichi、Nochi Tomonori、Aso Hisashi
  • Journal Title: Veterinary Research Volume: 49 Issue: 1 Pages: 22-22
  • DOI: 10.1186/s13567-018-0517-y
  • NAID: 120006798140
  • Peer Reviewed / Open Access
• [Journal Article] Effects of a moderate-fat diet that is enriched with fish oil on intestinal lipid absorption in a senescence-accelerated prone mouse model (2018)
  • Author(s): Yamamoto Kazushi、Iwagaki Yui、Watanabe Kouichi、Nochi Tomonori、Aso Hisashi、Tsuduki Tsuyoshi
  • Journal Title: Nutrition Volume: 50 Pages: 26-35
  • DOI: 10.1016/j.nut.2017.10.015
  • Peer Reviewed / Open Access
• [Journal Article] Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells (2017)
  • Author(s): Paulraj Kanmani, Leonardo Albarra2018.00326in, Hisakazu Kobayashi, Hikaru Iida, Ryoya Komatsu, A.K.M. Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Seiya Makino, Hiroshi Kano, Tadao Saito, JulioVillen, Haruki Kitazawa
  • Journal Title: Molecular Immunology Volume: 93 Pages: 253-265
  • DOI: 10.1016/j.molimm.2017.07.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transcriptomic analysis of the innate antiviral immune response in porcine intestinal epithelial cells: influence of immunobiotic lactobacilli. (2017)
  • Author(s): Albarracin, L., H. Kobayashi, H. Iida, N. Sato, T. Nochi, H. Aso, S. Salva, S. Alvarez, H. Kitazawa, J. Villena
  • Journal Title: Front. Immunol. Volume: 8(57) Pages: 1-15
  • DOI: 10.3389/fimmu.2017.00057
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development of an in vitro immunobiotic evaluation system against rotavirus infection in bovine intestinal epitheliocytes (2017)
  • Author(s): Kobayashi, H., P. Kanmani, T. Ishizuka, A. Miyazaki, J. Soma, L. Albarracin, Y. Suda, T. Nochi, H. Aso, N. Iwabuchi, J-z. Xiao, T. Saito, J. Villena, H. Kitazawa
  • Journal Title: Beneficial Microbes Volume: 8 (2) Issue: 2 Pages: 309-321
  • DOI: 10.3920/bm2016.0155
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937: finding new biomarkers for the selection of antiinflammatory immunobiotics. (2016)
  • Author(s): Kobayashi, H., L. Albarracin, N. Sato, P. Kanmani, AKM H. Kober, W. Ikeda-Ohtsubo, Y. Suda, T. Nochi, H. Aso, S. Makino, H. Kano, S. Ohkawara, T. Saito, J. Villena, H. Kitazawa
  • Journal Title: Beneficial Microbes, Benef Microbes Volume: 7 Issue: 5 Pages: 769-782
  • DOI: 10.3920/bm2016.0095
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lactogenic hormones alter cellular and extracellular microRNA expression in bovine mammary epithelial cell culture (2016)
  • Author(s): Susumu Muroya, Tatsuro Hagi, Ataru Kimura, Hisashi Aso, Masatoshi Matsuzaki and Masaru Nomura
  • Journal Title: Journal of Animal Science and Biotechnology Volume: 7 Issue: 1 Pages: 8-8
  • DOI: 10.1186/s40104-016-0068-x
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dynamic Metabolic Regulation by a Chromosome Segment from a Wild Relative During Fruit Development in a Tomato Introgression Line, IL8-3 (2016)
  • Author(s): Hiroki Ikeda, Tomoki Shibuya, Shunsuke Imanishi, Hisashi Aso, Manabu Nishiyama and Yoshinori Kanayama
  • Journal Title: Plant and Cell Physiology Volume: 57 Issue: 6 Pages: 1257-1270
  • DOI: 10.1093/pcp/pcw075
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mycoplasma pneumonia of swine (MPS) resistance and immune characteristics of pig lines generated by crossing an MPS pulmonary lesion selected Landrace line and a highly immune capacity selected Large White line. (2016)
  • Author(s): Borjigin L, Shimazu T, Katayama Y, Watanabe K, Kitazawa H, Roh SG, Aso H, Katoh K, Uchida T, Suda Y, Sakuma A, Nakajo M, Suzuki K
  • Journal Title: Animal Science Journal Volume: 印刷中 Issue: 8 Pages: 972-981
  • DOI: 10.1111/asj.12529
  • Peer Reviewed / Open Access
• [Journal Article] Immunogenic properties of Landrace pigs selected for resistance to mycoplasma pneumonia of swine. (2016)
  • Author(s): Borjigin L, Shimazu T, Katayama Y, Watanabe K, Kitazawa H, Roh S, Aso H, Katoh K, Uchida T, Suda Y, Sakuma A, Nakajo M and Suzuki K
  • Journal Title: Animal Science Journal Volume: 印刷中 Issue: 5 Pages: 638-645
  • DOI: 10.1111/asj.12471
  • Peer Reviewed / Open Access
• [Journal Article] Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells. (2016)
  • Author(s): Kohichiro Tsukida, Takuya Takahashi, Hikaru Iida, Paulraj Kanmani, Yoshihito Suda, Tomonori Nochi, Shuichi Ohwada, Hisashi Aso, Sou Ohkawara, Seiya Makino, Hiroshi Kano, Tadao Saito, Julio Villena, and Haruki Kitazawa
  • Journal Title: BMC Immunology Volume: 17 Issue: 1 Pages: 1-18
  • DOI: 10.1186/s12865-016-0160-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immunogenic properties of Landrace pigs selected for resistance to mycoplasma pneumonia of swine. (2016)
  • Author(s): Borjigin L, Shimazu T, Katayama Y, Li M, Satoh T, Watanabe K, Kitazawa H, Roh S, Aso H, Katoh K, Uchida T, Suda Y, Sakuma A, Nakajo M and Suzuki K
  • Journal Title: Animal Science Journal Volume: 印刷中 Issue: 3 Pages: 321-329
  • DOI: 10.1111/asj.12440
  • Peer Reviewed / Open Access
• [Journal Article] 2.Immunobiotic Bifidobacteria Strains Modulate Rotavirus Immune Response in Porcine Intestinal Epitheliocytes via Pattern Recognition Receptor Signaling. (2016)
  • Author(s): Ishizuka T, Kanmani P, Kobayashi H, Miyazaki A, Soma J, Suda Y, Aso H, Nochi T, Iwabuchi N, Xiao JZ, Saito T, Villena J, Kitazawa H.
  • Journal Title: PLoS One. Volume: 11 Issue: 3 Pages: 1-19
  • DOI: 10.1371/journal.pone.0152416
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cyclophilin A is a new M cell marker of bovine intestinal epithelium. (2016)
  • Author(s): Tetsuya Hondo , Shunsuke Someya , Yuya Nagasawa , Shunsuke Terada, Hitoshi Watanabe, Xiangning Chen, Kouichi Watanabe, Shyuichi Ohwada, Haruki Kitazawa, Michael T. Rose, Tomonori Nochi, Hisashi Aso
  • Journal Title: Cell and Tissue Research Volume: 364 Issue: 3 Pages: 585-597
  • DOI: 10.1007/s00441-015-2342-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Simultaneous analysis of serotonin, tryptophan and tryptamine levels in common fresh fruits and vegetables in Japan using fluorescence HPLC (2016)
  • Author(s): Jahidul Islam, Hitoshi Shirakawa, Thomas Kim Nguyen, Hisashi Aso, Michio Komai
  • Journal Title: Food Bioscience Volume: 13 Pages: 56-59
  • DOI: 10.1016/j.fbio.2015.12.006
  • Peer Reviewed / Open Access
• [Presentation] 末梢セロトニンの褐色脂肪細胞活性化による抗肥満作用 (2018)
  • Author(s): 田口輝明・甲藤士郎・田中孝太朗・庄 涛・吉村 梢・渡邊康一・野地智法・麻生 久
  • Organizer: 日本畜産学会第124回大会
• [Presentation] 初産牛における乳汁中の体細胞数と炎症誘起因子シクロフィリンA濃度との相関解析 (2018)
  • Author(s): 海野 剛・浦川めぐみ・庄 涛・田中孝太朗・吉村 梢・佐藤佑子・伊藤 愛・小林宏子・渡邊康一・野地智法・麻生 久
  • Organizer: 日本畜産学会第124回大会
• [Presentation] ブタにおける二次リンパ組織の形態形成機序の解明 (2018)
  • Author(s): 古川睦実・伊藤 駿・鈴木俊一・淵本大一郎・渡邊康一・麻生 久・野地智法
  • Organizer: 日本畜産学会第124回大会
• [Presentation] Development of immune and microbial environments is independently regulated in the mammary gland (2017)
  • Author(s): Niimi K., Usami K., Aso H., Nochi T.
  • Organizer: 日本免疫学会第46回学術集会
• [Presentation] Important role of the immunological network between large intestine and mammary gland in breastfeeding (2017)
  • Author(s): Usami K., Niimi K., Kamioka M., Kiyono H., Aso H., Nochi T.
  • Organizer: 日本免疫学会第46回学術集会
• [Presentation] プロバイオティクス枯草菌によるウシ乳房炎の発症軽減効果 (2017)
  • Author(s): 浦川めぐみ・佐藤秀俊・庄 涛・田口輝明・海野 剛・田中孝太朗・甲藤士郎・吉村 梢・小林宏子・伊藤 愛・高田直和・芦田延久・大友直樹・渡邊康一・野地智法・麻生 久
  • Organizer: 日本乳房炎研究会第22回学術集会
• [Presentation] 二次リンパ組織の形態形成に関するブタ胎仔を用いた組織学的研究 (2017)
  • Author(s): 古川睦実、伊藤 駿、盛田彰太郎、鈴木俊一、淵本大一郎、中井美智子、大西 彰、新實香奈枝、宇佐美克紀、佐々木志保、渡邊康一、麻生 久、野地智法
  • Organizer: 日本獣医学会第160回学術集会
• [Presentation] X-SCIDモデルブタ（IL2Rg欠損ブタ）の各種臓器内に認められるＴ細胞およびＢ細胞分布に関する組織学的研究 (2017)
  • Author(s): 伊藤 駿、古川睦実、盛田彰太郎、鈴木俊一、淵本大一郎、中井美智子、大西 彰、新實香奈枝、宇佐美克紀、佐々木志保、渡邊康一、麻生 久、野地智法
  • Organizer: 日本獣医学会第160回学術集会
• [Presentation] Effects of common microbial ligands for TLRs on adipogenesis and inflammation in porcine adipocytes (2017)
  • Author(s): 井形愛美・K.AKM Humayun・Tada A.・A. Leonardo・Ikeda-Ohtsubo W.・Aso H.・Yoda K.・Miyazawa K.・He Fang・Saito T.・Villena J.・Kitazawa H.
  • Organizer: 日本畜産学会第123回大会
• [Presentation] Dispensable role of indigenous bacteria in developing the mammary gland functions (2017)
  • Author(s): Niimi K.・Usami K.・Furukawa M.・Sasaki S.・Ito S.・Banba Y.・Watanabe K.・Aso H.・Nochi T.
  • Organizer: 日本畜産学会第123回大会
• [Presentation] 末梢セロトニンの骨格筋線維型移行および代謝亢進作用の解明 (2017)
  • Author(s): 甲藤士郎・田口輝明・田中孝太朗・庄 涛・吉村 梢・渡邊康一・野地智法・麻生 久
  • Organizer: 日本畜産学会第123回大会
• [Presentation] Development of advanced evaluation system of anti-disease immunobiotics (2017)
  • Author(s): Komatsu R.・Iida H.・K.AKM Humayun・Albarracin Leonardo 3・Suda Y.・Ikeda-Ohtsubo W.・Aso H.・Iwabuchi N.・Xiao Jin-zhong・Saito T.・Villena J.・Kitazawa H.
  • Organizer: 日本畜産学会第123回大会
• [Presentation] 授乳期特有の乳腺免疫の発達に関わるＴ細胞の役割とは (2017)
  • Author(s): 宇佐美克紀・新實香奈枝・古川睦実・佐々木志保・伊藤 駿・馬場柚里・渡邊康一・麻生 久・野地智法
  • Organizer: 東北畜産学会第67回大会
• [Presentation] 授乳期の乳腺の機能形成おける役割 (2017)
  • Author(s): 佐々木志保・新實香奈枝・宇佐美克紀・古川睦実・伊藤 駿・馬場柚里・渡邊康一・麻生 久・野地智法
  • Organizer: 東北畜産学会第67回大会
• [Presentation] マウス腸管上皮M 細胞におけるアルドラーゼA受容体の探索 (2017)
  • Author(s): 鈴木 京・長澤裕哉・田中孝太朗・庄 涛・田口輝明・北澤春樹・渡邊康一・野地智法・麻生 久
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸市、神戸大学
• [Presentation] 乳腺と大腸をつなぐ免疫ネットワークの解明 (2017)
  • Author(s): 宇佐美克紀・新實香奈枝・古川睦実・佐々木志保・伊藤 駿・渡邊康一・麻生 久・野地智法
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸市、神戸大学
• [Presentation] ウシ血中樹状細胞の精製法確立と枯草菌給与による活性化機構の解明 (2017)
  • Author(s): 庄 涛・浦川めぐみ・鈴木 京・田口輝明・佐藤秀俊・高田直和・及川俊徳・小林宏子・伊藤 愛・芦田延久・今林寛和・渡邊康一・野地智法・麻生 久
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸市、神戸大学
• [Presentation] 枯草菌の給与が乳牛の血中栄養因子および免疫因子に与える影響 (2017)
  • Author(s): 浦川めぐみ・佐藤秀俊・庄 涛・鈴木 京・田口輝明・小林宏子・伊藤 愛・高田 直和・及川俊徳・芦田延久・今林寛和・渡邊康一・野地智法・麻生 久
  • Organizer: 日本畜産学会第122回大会
  • Place of Presentation: 神戸市、神戸大学
• [Presentation] 黄色ブドウ球菌特異的IgG 抗体はSortase A非依存的に細胞増殖を阻害する (2017)
  • Author(s): 古川睦実・米山 裕・秦 英司・安藤太助・林 智人・菊 佳男・長澤裕哉・渡邊康一・麻生 久・野地智法
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台市、仙台国際センター
• [Presentation] 黄色ブドウ球菌（SA）の死菌接種によって産生されるポリクローナル抗体はSAに対する増殖阻害効果を有する (2016)
  • Author(s): 古川睦実・米山 裕・秦 英司・岩野英知・樋口豪紀・安藤太助・佐藤美佳・林 智人・菊 佳男・長澤裕哉・新實香奈枝・宇佐美克紀・佐々木志保・伊藤 駿・渡邊康一・麻生 久・野地智法
  • Organizer: 第6回家畜感染症学会
  • Place of Presentation: 福岡市、九州大学西新プラザ
• [Presentation] フローサイトメトリーを用いた授乳期の乳腺に認められるIgA産生形質細胞の性状解析 (2016)
  • Author(s): 宇佐美克紀・新實香奈枝・古川睦実・藤田勇気・阿部未来・渡邊康一・麻生 久・野地智法
  • Organizer: 第159回日本獣医学会
  • Place of Presentation: 藤沢市、日本大学生物資源科学部
• [Presentation] 褐色脂肪細胞の活性化による経口セロトニンの抗肥満作用 (2016)
  • Author(s): 田口輝明・日高湧介・海野 剛・對木陽淳・陳 香凝・北澤春樹・渡邊康一・野地智法・麻生 久
  • Organizer: 第66回東北畜産学会大会
  • Place of Presentation: 盛岡市、いわて県民情報交流センター
• [Presentation] 枯草菌給与によるウシ分娩前後における血中樹状細胞の機能解析 (2016)
  • Author(s): 庄 涛・浦川めぐみ・佐藤秀俊・高田直和・及川俊徳・小林宏子・伊藤 愛・芦田延久・今林寛和・渡邊康一・野地智法・麻生 久
  • Organizer: 第66回東北畜産学会大会
  • Place of Presentation: 盛岡市、いわて県民情報交流センター
• [Presentation] 黄色ブドウ球菌のin vitroでの増殖阻害を可能にするウシポリクローナル抗体の作製 (2016)
  • Author(s): 古川睦実・米山 裕・秦 英司・岩野英知・樋口豪紀・安藤太助・佐藤美佳・林 智人・菊 佳男・長澤裕哉・新實香奈枝・宇佐美克紀・佐々木志保・伊藤 駿・渡邊康一・麻生 久・野地智法
  • Organizer: 第66回東北畜産学会大会
  • Place of Presentation: 盛岡市、いわて県民情報交流センター
• [Book] Energy homeostasis by peripheral serotonin (2017)
  • Author(s): Hitoshi Watanabe, Michael Rose, Yoshinori Kanayama, Hitoshi Shirakawa and Hisashi Aso
  • Total Pages: 305
  • Publisher: InTech Team
  • ISBN: 9789535152903
• [Remarks] 東北大学大学院農学研究科機能形態学分野
  • URL: http://www.agri.tohoku.ac.jp/keitai/index-j.html
• [Remarks] 東北大学大学院農学研究科食と農免疫国際教育研究センター
  • URL: http://www.agri.tohoku.ac.jp/cfai/index.html