Development of vector-borne disease control method using venom expression parasite

• Project/Area Number: 16K15035
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: Fukumoto Shinya 帯広畜産大学, 原虫病研究センター, 准教授 (50376422)
• Research Collaborator: Aiuchi Daigo
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: マラリア原虫 / ハマダラカ / ヴェノム / マラリア / ベクター / 感染症

Outline of Final Research Achievements

In vector-borne infection measures such as mosquitoes, vector control by insecticides is the most important strategy. However, the use of insecticides has various problems such as acquiring resistance and side effects. In this study, we focused on Venom, a natural poison possessed by natural enemies. We succeeded in producing venom-expressing malaria parasites using melittin as a model. In addition, we succeeded in developing a new gene recombination method for rodent malaria parasite, and it has facilitated the production of multiple mutants that were difficult to date. It was also suggested that venom-expressing parasite could be applied to vaccine development.

Academic Significance and Societal Importance of the Research Achievements

本研究成果により蚊感染ステージ特異的にヴェノム発現原虫を作製可能であることが確認された。この結果は、今後の更なる研究の継続と進展により、現在までの主流であったケミカル殺虫剤に依存しない蚊への対策法の確立と全く新たな概念に基づくワクチンの開発に繋がることが示唆された。
また本研究で新たに開発された齧歯類マラリア原虫遺伝子改変技術はマラリア研究の基底スピード向上に繋がるものであり、マラリア根絶に向け寄与可能な結果であると考えられる。

Research Products

• [Journal Article] High frequencies of F1534C and V1016I kdr mutations and association with pyrethroid resistance in Aedes aegypti from Somgande; (Ouagadougou), Burkina Faso (2019)
  • Author(s): Aboubacar Sombie, Erisha Saiki, Felix Yameogo, Tatsuya Sakurai, Takahiro Shirozu, Shinya Fukumoto, Antoine Sanon, David Weetman, Philip J. McCall, Hirotaka Kanuka, Athanase Badolo
  • Journal Title: Tropical Medicine and Health Volume: 47 Issue: 1 Pages: 2-2
  • DOI: 10.1186/s41182-018-0134-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Adeno-Associated Virus as an Effective Malaria Booster Vaccine Following Adenovirus Priming (2019)
  • Author(s): Yusuf Y, Yoshii T, Iyori M, Yoshida K, Mizukami H, Fukumoto S, Yamamoto DS, Alam A, Emran TB, Amelia F, Islam A, Otsuka H, Takashima E, Tsuboi T, Yoshida S.
  • Journal Title: Frontiers in Immunology Volume: 10 Pages: 730-730
  • DOI: 10.3389/fimmu.2019.00730
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of a bsd-blasticidin selection system in Plasmodium berghei (2018)
  • Author(s): Akira Soga, Mami Ko-ketsu, Shinya Fukumoto
  • Journal Title: FEBS Letters Volume: 592 Issue: 11 Pages: 1847-1855
  • DOI: 10.1002/1873-3468.13100
  • Peer Reviewed
• [Journal Article] High efficacy in vitro selection procedure for generating transgenic parasites of Plasmodium berghei using an antibiotic toxic to rodent hosts (2017)
  • Author(s): Soga Akira、Bando Hironori、Ko-ketsu Mami、Masuda-Suganuma Hirono、Kawazu Shin-ichiro、Fukumoto Shinya
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 4001-4001
  • DOI: 10.1038/s41598-017-04244-0
  • Peer Reviewed / Open Access
• [Journal Article] Molecular characterization of Fasciola flukes obtained from wild sika deer and domestic cattle in Hokkaido, Japan (2017)
  • Author(s): Ichikawa-Seki Madoka、Shiroma Tomoko、Kariya Tatsuya、Nakao Ryo、Ohari Yuma、Hayashi Kei、Fukumoto Shinya
  • Journal Title: Parasitology International Volume: 66 Issue: 5 Pages: 519-521
  • DOI: 10.1016/j.parint.2017.04.005
  • Peer Reviewed / Open Access
• [Presentation] Development of high efficacy in vitro drug selection systems for generating transgenic parasite of Plasmodium berghei (2018)
  • Author(s): Akira Soga , Mami Ko-ketsu, Shinya Fukumoto
  • Organizer: 14th International Congress of Parasitology
  • Int'l Joint Research
• [Presentation] RNA結合タンパク質PbRBP1のマラリア原虫蚊体内ステージにおける機能解析 (2018)
  • Author(s): 曽賀晃、纐纈摩美、福本晋也
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] 宿主毒性薬剤選択系を用いたPlasmodiuim berghei遺伝子ターゲティング法の確立 (2017)
  • Author(s): 曽賀晃、纐纈摩美、福本晋也
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Development of high efficacy in vitro drug selection method for sequential genetic manipulation of Plasmodium berghei (2017)
  • Author(s): Akira Soga ; Mami Ko-ketsu ; Shinya Fukumoto
  • Organizer: WAAVP2017
  • Int'l Joint Research
• [Presentation] ネズミマラリア原虫Plasmodium bergheiにおけるin vitro薬剤選択法の改良 (2017)
  • Author(s): 曽賀晃、纐纈摩美、福本晋也
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] 宿主毒性薬剤選択系を用いたネズミマラリア原虫効率的遺伝子操作法の開発 (2017)
  • Author(s): 曽賀晃、纐纈摩美、福本晋也
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] 哺乳類宿主毒性抗生剤を利用したPlasmodium berghei多重変異体作製技術の開発 (2016)
  • Author(s): 曽賀晃、纐纈摩美、福本晋也
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2016-11-30
• [Presentation] In vitro哺乳動物毒性マーカーシステムの応用によるマウスマラリアモデル 多重変異体選択技術の開発 (2016)
  • Author(s): 曽賀晃、纐纈摩美、福本晋也
  • Organizer: 第159回日本獣医学会
  • Place of Presentation: 日本大学（神奈川県・藤沢市）
  • Year and Date: 2016-09-06