| Project/Area Number |
16K15103
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
Otomo Kohei 北海道大学, 電子科学研究所, 助教 (40547204)
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| Co-Investigator(Renkei-kenkyūsha) |
NEMOTO Tomomi 北海道大学, 電子科学研究所, 教授 (50291084)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 二光子顕微鏡 / 超解像顕微鏡 / STED顕微鏡 / スピニングディスク顕微鏡 / in vivoイメージング / バイオイメージング / 二光子顕微鏡法 / 超解像顕微鏡法 / スピニングディスク / ニポウディスク |
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| Outline of Final Research Achievements |
This study aimed at developing novel two-photon excitation microscopy which possesses the spatial resolution overcoming diffraction limits and millisecond temporal resolution to visualize living lipid microdomain in real time. To accomplish the purpose, we further improved both two-photon excitation stimulated emission depletion (STED) microscopy and multi-point scanning two-photon excitation microscopy, which we had developed so far. Although we have not developed the initially planned microscopy system which combined both technologies, resolutions and utilities of respective microscopy systems were successfully improved. Especially, the latter multi-point scanning system could be utilized for general purposes, thus we started to open this system as the equipment of Nikon imaging center at Hokkaido University.
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