Development of fluorescent probe for detection of reductive stress

• Project/Area Number: 16K15109
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Kyushu University
• Principal Investigator: Yamada Ken-ichi 九州大学, 薬学研究院, 教授 (60346806)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 薬学 / 分析科学 / ストレス

Outline of Final Research Achievements

In development of many oxidative disease, change of redox reaction have been reported to be involved. Here, in this study, we aimed to develop a detection probe for reductive stress. We herein designed a mitochondrial targeting profluorescent nitroxide probe for the detection of reductive stress. We demonstrated that this probe can detect an excess amount of electron donating compound in living cell, which causes the mitochondrial reductive stress.

Research Products

• [Journal Article] Non-invasive imaging of the levels and effects of glutathione on the redox status of mouse brain using electron paramagnetic resonance imaging (2017)
  • Author(s): Miho C. Emoto, Yuta Matsuoka, Ken-ichi Yamada, Hideo Sato-Akaba, Hirotada G. Fujii
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 485 Issue: 4 Pages: 802-806
  • DOI: 10.1016/j.bbrc.2017.02.134
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Lipophilic tetraphenylphosphonium derivatives enhance radiation-induced cell killing via inhibition of mitochondrial energy metabolism in tumor cells (2017)
  • Author(s): Yasui H, Yamamoto K, Suzuki M, Sakai Y, Bo T, Nagane M, Nishimura E, Yamamori T, Yamasaki T, Yamada K, Inanami O
  • Journal Title: Cancer Letters Volume: 390 Pages: 160-167
  • DOI: 10.1016/j.canlet.2017.01.006
  • Peer Reviewed / Open Access
• [Presentation] アスコルビン酸の迅速かつ高感度な検出に向けた長波長蛍光ニトロキシド化合物の開発 (2016)
  • Author(s): 松岡 悠太, 大久保 敬, 福住 俊一, 山田 健一
  • Organizer: 第29回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 京都市
  • Year and Date: 2016-09-02
• [Presentation] Synthesis and evaluation of 2,6-substituted TEMPO derivatives (2016)
  • Author(s): Yuta Matsuoka, Toshihide Yamasaki, Ken-ichi Yamada
  • Organizer: 第16回 日本NO学会学術集会、第9回国際NO学会
  • Place of Presentation: 仙台市
  • Year and Date: 2016-05-20
  • Int'l Joint Research