| Project/Area Number |
16K15118
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
若杉 光生 金沢大学, 薬学系, 准教授 (80345595)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ヌクレオチド除去修復 / 紫外線 / DNA損傷 / 色素性乾皮症 / 遺伝子 / 遺伝学 / 癌 / 薬学 |
|---|
| Outline of Final Research Achievements |
We recently showed that mammalian cells slowly remove UV-induced 6-4 photoproducts from their genome under the nucleotide excision repair (NER)-defective condition. In this study, we have tried to uncover the mechanism of the NER-independent reaction in human cells. We have found that the non-canonical repair reaction requires XPC, but not DDB, both of which are well-known NER factors involved in a damage recognition step. We further found that a proteasomal activity is also required for this reaction only when NER-deficient cells have functional DDB, suggesting the implication of proteasomal degradation mediated by Cul4-DDB1 E3 ligase.
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