Analysis of the mechanism of a novel DNA repair reaction in mammalian cells
| Project/Area Number |
16K15118
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
若杉 光生 金沢大学, 薬学系, 准教授 (80345595)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ヌクレオチド除去修復 / 紫外線 / DNA損傷 / 色素性乾皮症 / 遺伝子 / 遺伝学 / 癌 / 薬学 |
|---|
| Outline of Final Research Achievements |
We recently showed that mammalian cells slowly remove UV-induced 6-4 photoproducts from their genome under the nucleotide excision repair (NER)-defective condition. In this study, we have tried to uncover the mechanism of the NER-independent reaction in human cells. We have found that the non-canonical repair reaction requires XPC, but not DDB, both of which are well-known NER factors involved in a damage recognition step. We further found that a proteasomal activity is also required for this reaction only when NER-deficient cells have functional DDB, suggesting the implication of proteasomal degradation mediated by Cul4-DDB1 E3 ligase.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Rapid G0/1 transition and cell cycle progression in CD8+ T Cells compared to CD4+ T Cells following in vitro stimulation2017
Author(s)
Mishima, T., Fukaya, S., Toda, S., Ando, Y., Matsunaga, T. and Inobe, M.
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Journal Title
Microbiology and Immunology
Volume: 印刷中
Issue: 5
Pages: 168-175
DOI
NAID
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Peer Reviewed
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[Book] 光と生命の事典2017
Author(s)
日本光生物学協会 光と生命の事典 編集委員会 編
Total Pages
436
Publisher
朝倉書店
Related Report
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