| Project/Area Number |
16K15150
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
永瀬 久光 岐阜薬科大学, 薬学部, 教授 (40141395)
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| Co-Investigator(Renkei-kenkyūsha) |
IKAWA MASAHITO 大阪大学, 微生物病研究所, 教授 (20304066)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | リポカリン / メタボリック症候群 / CRISPR/Cas9 / ノックアウトマウス / 栄養化学 / メタボリックシンドローム / 遺伝子欠損マウス |
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| Outline of Final Research Achievements |
In our current study, we generated C8 gamma-deficient mice using the CRISPR/Cas9 system and investigated the physiological impact of C8 gamma in adipocyte differentiation and glycolipid metabolism using the mice. Seven deletion-mutant mice were obtained and one of them (C8g-KO#1) was observed 302 bp DNA deletion from Exon5 to the intron portion of the downstream. In addition, mRNA expression of C8gamma was not detected in any tissue of homo C8g-KO#1. The deficient of C8 gamma had no effect on lipidemia and body weight gain induced by high-fat diet, but promoted the reduction of brown adipocyte mass. Our findings suggest that C8 gamma may be involved in brown adipocyte differentiation in obesity development.
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