• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Physiological impact of C8 gamma in adipocyte differentiation and glycolipid metabolism

Research Project

Project/Area Number 16K15150
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental and hygienic pharmacy
Research InstitutionGifu Pharmaceutical University

Principal Investigator

Nakanishi Tsuyoshi  岐阜薬科大学, 薬学部, 准教授 (50303988)

Co-Investigator(Kenkyū-buntansha) 永瀬 久光  岐阜薬科大学, 薬学部, 教授 (40141395)
Co-Investigator(Renkei-kenkyūsha) IKAWA MASAHITO  大阪大学, 微生物病研究所, 教授 (20304066)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsリポカリン / メタボリック症候群 / CRISPR/Cas9 / ノックアウトマウス / 栄養化学 / メタボリックシンドローム / 遺伝子欠損マウス
Outline of Final Research Achievements

In our current study, we generated C8 gamma-deficient mice using the CRISPR/Cas9 system and investigated the physiological impact of C8 gamma in adipocyte differentiation and glycolipid metabolism using the mice. Seven deletion-mutant mice were obtained and one of them (C8g-KO#1) was observed 302 bp DNA deletion from Exon5 to the intron portion of the downstream. In addition, mRNA expression of C8gamma was not detected in any tissue of homo C8g-KO#1. The deficient of C8 gamma had no effect on lipidemia and body weight gain induced by high-fat diet, but promoted the reduction of brown adipocyte mass. Our findings suggest that C8 gamma may be involved in brown adipocyte differentiation in obesity development.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] Peking University(China)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Potential Interference of Oil Vehicles on Genital Tubercle Development during the Fetal Period in ICR Mice2018

    • Author(s)
      Nishioka Y, Tamai K, Onda M, Hiromori Y, Kimura T, Hu J, Nagase H, Nakanishi T.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 41 Issue: 2 Pages: 266-271

    • DOI

      10.1248/bpb.b17-00830

    • NAID

      130006329273

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Occurrence of fibrates and their metabolites in source and drinking water in Shanghai and Zhejiang, China2017

    • Author(s)
      Akiko Ido, Youhei Hiromori, Liping Meng, Haruki Usuda, Hisamitsu Nagase, Min Yang, Jianying Hu and Tsuyoshi Nakanishi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 45931-45931

    • DOI

      10.1038/srep45931

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Germline recombination in a novel Cre transgenic line, Prl3b1-cre mouse.2016

    • Author(s)
      Al-Soudy AS, Hiromori Y, Mizuno S, Hasegawa Y, Shawki HH, Katoh MC, Basha WA, Ibrahim AE, El-Shemy HA, Iseki Hi, Yoshiki A, Nagase H, Nakanishi T, Takahashi S, Oishi H, Sugiyama F.
    • Journal Title

      Genesis.

      Volume: 54(7) Issue: 7 Pages: 389-97

    • DOI

      10.1002/dvg.22944

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] フルタミドによる肝臓の糖脂質代謝異常の誘発とその分子メカニズムの解明2018

    • Author(s)
      椿原伊織、髙木 康平、中西 剛、永瀬久光
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 核内受容体を介した有機スズ化合物の生態影響2017

    • Author(s)
      中西 剛
    • Organizer
      第23 回ヒ素シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Occurrence of fibrates and their metabolites in source and drinking water in Shanghai and Zhejiang, China, 20172017

    • Author(s)
      Ido A, Hiromori Y, Meng L, Usuda H, Yang M, Hu J, Nakanishi T, Nagase H
    • Organizer
      Japan/Korea Symposium on Pharmaceutical Health Science and Environmental Toxicology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 抗アンドロゲン剤による肝臟の糖代謝異常を伴う脂肪蓄積の亢進と脂肪肝の誘発2016

    • Author(s)
      高木康平 他4名
    • Organizer
      第43回日本毒性学会学術年会
    • Place of Presentation
      名古屋市
    • Related Report
      2016 Research-status Report
  • [Presentation] フタル酸ジエチルヘキシル(DHEP)経口曝露が免疫組織に及ぼす影響2016

    • Author(s)
      廣森洋平 他3名
    • Organizer
      第43回日本毒性学会学術年会
    • Place of Presentation
      名古屋市
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-12-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi