Physiological impact of C8 gamma in adipocyte differentiation and glycolipid metabolism

• Project/Area Number: 16K15150
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental and hygienic pharmacy
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: Nakanishi Tsuyoshi 岐阜薬科大学, 薬学部, 准教授 (50303988)
• Co-Investigator(Kenkyū-buntansha): 永瀬 久光 岐阜薬科大学, 薬学部, 教授 (40141395)
• Co-Investigator(Renkei-kenkyūsha): IKAWA MASAHITO 大阪大学, 微生物病研究所, 教授 (20304066)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リポカリン / メタボリック症候群 / CRISPR/Cas9 / ノックアウトマウス / 栄養化学 / メタボリックシンドローム / 遺伝子欠損マウス

Outline of Final Research Achievements

In our current study, we generated C8 gamma-deficient mice using the CRISPR/Cas9 system and investigated the physiological impact of C8 gamma in adipocyte differentiation and glycolipid metabolism using the mice. Seven deletion-mutant mice were obtained and one of them (C8g-KO#1) was observed 302 bp DNA deletion from Exon5 to the intron portion of the downstream. In addition, mRNA expression of C8gamma was not detected in any tissue of homo C8g-KO#1. The deficient of C8 gamma had no effect on lipidemia and body weight gain induced by high-fat diet, but promoted the reduction of brown adipocyte mass. Our findings suggest that C8 gamma may be involved in brown adipocyte differentiation in obesity development.

Research Products

• [Int'l Joint Research] Peking University(China)
• [Journal Article] Potential Interference of Oil Vehicles on Genital Tubercle Development during the Fetal Period in ICR Mice (2018)
  • Author(s): Nishioka Y, Tamai K, Onda M, Hiromori Y, Kimura T, Hu J, Nagase H, Nakanishi T.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 41 Issue: 2 Pages: 266-271
  • DOI: 10.1248/bpb.b17-00830
  • NAID: 130006329273
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Occurrence of fibrates and their metabolites in source and drinking water in Shanghai and Zhejiang, China (2017)
  • Author(s): Akiko Ido, Youhei Hiromori, Liping Meng, Haruki Usuda, Hisamitsu Nagase, Min Yang, Jianying Hu and Tsuyoshi Nakanishi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 45931-45931
  • DOI: 10.1038/srep45931
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Germline recombination in a novel Cre transgenic line, Prl3b1-cre mouse. (2016)
  • Author(s): Al-Soudy AS, Hiromori Y, Mizuno S, Hasegawa Y, Shawki HH, Katoh MC, Basha WA, Ibrahim AE, El-Shemy HA, Iseki Hi, Yoshiki A, Nagase H, Nakanishi T, Takahashi S, Oishi H, Sugiyama F.
  • Journal Title: Genesis. Volume: 54(7) Issue: 7 Pages: 389-97
  • DOI: 10.1002/dvg.22944
  • Peer Reviewed / Int'l Joint Research
• [Presentation] フルタミドによる肝臓の糖脂質代謝異常の誘発とその分子メカニズムの解明 (2018)
  • Author(s): 椿原伊織、髙木 康平、中西 剛、永瀬久光
  • Organizer: 日本薬学会第138年会
• [Presentation] 核内受容体を介した有機スズ化合物の生態影響 (2017)
  • Author(s): 中西 剛
  • Organizer: 第23 回ヒ素シンポジウム
  • Invited
• [Presentation] Occurrence of fibrates and their metabolites in source and drinking water in Shanghai and Zhejiang, China, 2017 (2017)
  • Author(s): Ido A, Hiromori Y, Meng L, Usuda H, Yang M, Hu J, Nakanishi T, Nagase H
  • Organizer: Japan/Korea Symposium on Pharmaceutical Health Science and Environmental Toxicology
  • Int'l Joint Research
• [Presentation] 抗アンドロゲン剤による肝臟の糖代謝異常を伴う脂肪蓄積の亢進と脂肪肝の誘発 (2016)
  • Author(s): 高木康平 他4名
  • Organizer: 第43回日本毒性学会学術年会
  • Place of Presentation: 名古屋市
• [Presentation] フタル酸ジエチルヘキシル（DHEP）経口曝露が免疫組織に及ぼす影響 (2016)
  • Author(s): 廣森洋平 他3名
  • Organizer: 第43回日本毒性学会学術年会
  • Place of Presentation: 名古屋市