Construction of a model for intestinal toxicity test using human and monkey iPS cells and search for toxic biomarker
| Project/Area Number |
16K15164
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Nagoya City University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IWAO Takahiro 名古屋市立大学, 大学院薬学研究科, 准教授 (50581740)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ヒトiPS細胞 / カニクイザルiPS細胞 / 腸管上皮細胞 / 腸管オルガノイド / 抗がん剤 / 細胞毒性 / 腸管毒性試験 / サルiPS細胞 / 低分子化合物 / 分化誘導 / 毒性試験 / 分化 / 薬学 / iPS細胞 / オルガノイド |
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| Outline of Final Research Achievements |
In the differentiation of human iPS cells into enterocytes, the mRNA expression levels of intestinal markers and pharmacokinetic-related factors were increased by addition of small molecule compounds. On the other hand, these mRNA expression levels were increased by high molecule compounds. These results suggested that these compounds contribute obtain of intestinal functions.
Intestinal organoids were useful as a toxicity evaluation system, because dose-dependent cytotoxicity was observed by an anti-cancer drug 5-FU. The mRNA expression of inflammatory cytokines and markers of regenerative intestinal epithelial stem cells was increased by 5-FU. These results suggested that these expression changes are promising as cytotoxic markers.
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Report
(3 results)
Research Products
(29 results)
