Creation of single cell and real-time observation technology of virus infection based on genome modification

• Project/Area Number: 16K15331
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: WATANABE Eri 京都府立医科大学, 医学(系)研究科(研究院), 助教 (20433253)
• Co-Investigator(Renkei-kenkyūsha): MAZDA Osam 京都府立医科大学, 医学研究科, 教授 (00271164)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 臨床微生物学 / ウィルス感染 / ウイルス感染 / センサー細胞 / ウイルス / iPS細胞 / 感染 / IPS1

Outline of Final Research Achievements

In virus infection experiments, cells derived from humans matching the target virus tropism can sometimes not be supplied as experimental material. In that case, infection experiments are often carried out using a cultured cell line that is not a natural host, or a model system using related virus species. On the other hand, as a method of detecting virus infection, a retrospective proof method is mainstream. However, it is difficult to observe in live individual cells in real time, and it is not easy to detect infected virus quickly, highly sensitively and precisely.　In this study, we aimed to establish cells (virus sensor cells) that can observe viral infection in real time in cells close to the natural host using chromosome modification technology.