Development of the therapy for allergic airway hypersensitivity by using a phospholipid liposome

• Project/Area Number: 16K15455
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Respiratory organ internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: SHIBUYA Akira 筑波大学, 生命領域学際研究センター, 教授 (80216027)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 喘息

Outline of Final Research Achievements

Bronchial asthma is characterized by reversible bronchial obstruction, airway inflammation and airway hypersensitivity. Since some patients with this disease show resistance to the steroid therapy, the development of a new therapy for this disease is being expected. In this study, we aimed to clarify the effect of PC-liposome on airway hypersensitivity to methacholine. Mice that were treated i.n. with PC-liposome for 5 days 3 weeks after the start of induction of allergic asthma by HDM treatment showed amelioration of the airway hypersensitivity. Those mice treated with PC-liposome for 10 days 2 weeks after the start of induction of allergic asthma by HDM treatment showed no airway hypersensitivity. These results suggest a possibility that the therapy by PC-liposome is useful for the allergic asthma.

Research Products

• [Journal Article] Immunoreceptor CD300a on mast cells and dendritic cells regulates neutrophil recruitment in a murine model of sepsis (2016)
  • Author(s): Udayanga K G S, Nakamura Y, Nakahashi-Oda C, Shibuya A
  • Journal Title: Int Immunol Volume: 28 Issue: 12 Pages: 611-615
  • DOI: 10.1093/intimm/dxw047
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Allergin-1 inhibits TLR2-mediated mast cell activation and suppresses dermatitis (2016)
  • Author(s): Tsurusaki S, Tahara-Hanaoka S, Shibagaki S, Miyake S, Imai M, Shibayama S, Kubo M, Shibuya A
  • Journal Title: International Immunology Volume: 28(12) Issue: 12 Pages: 605-609
  • DOI: 10.1093/intimm/dxw046
  • NAID: 120007135029
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Improved protocol for the isolation of naive follicular dendritic cells (2016)
  • Author(s): Sato K, Honda SI, Shibuya A, Shibuya K
  • Journal Title: Mol Immunol Volume: 78 Pages: 140-145
  • DOI: 10.1016/j.molimm.2016.09.011
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Activation of murine invariant NKT cells promotes susceptibility to candidiasis by IL-10-induced modulation of phagocyte antifungal activity (2016)
  • Author(s): Haraguchi N, Kikuchi N, Morishima Y, Matsuyama M, Sakurai H, Shibuya A, Shibuya K, Taniguchi M, Ishii Y
  • Journal Title: Eur J Immunol Volume: 46 Issue: 7 Pages: 1691-1703
  • DOI: 10.1002/eji.201545987
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Soluble DNAM-1, as a predictive biomarker for acute graft-versus-host disease (2016)
  • Author(s): Kanaya M, Shibuya K, Hirochika R, Kanemoto M, Ohashi K, Okada M, Wagatsuma Y, Cho Y, Kojima H, Teshima T, Imamura M, Sakamaki H, Shibuya A
  • Journal Title: PloS One Volume: 11 Pages: 1-12
  • NAID: 120007129409
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Marginal zone B cells exacerbate endotoxic shock via interleukin-6 secretion induced by Fcα/μR-coupled TLR4 signalling (2016)
  • Author(s): Honda S, Sato K, Totsuka N, Fujiyama S, Fujimoto M, Miyake K, Nakahashi-Oda C, Tahara-Hanaoka S, Shibuya K, Shibuya A
  • Journal Title: Nat Commun Volume: 7 Issue: 1 Pages: 1-10
  • DOI: 10.1038/ncomms11498
  • NAID: 120007135503
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Increased soluble CD155 in the serum of cancer patients (2016)
  • Author(s): Iguchi-Manaka A, Okumura G, Kojima H, Cho Y, Hirochika R, Bando H, Sato T, Yoshikawa H, Hara H, Shibuya A, Shibuya K.
  • Journal Title: PLoS One Volume: 11(4) Issue: 4 Pages: 1-12
  • DOI: 10.1371/journal.pone.0152982
  • NAID: 120007135543
  • Peer Reviewed / Open Access
• [Presentation] 上皮の細胞死によるバリア組織の恒常性の制御 (2017)
  • Author(s): 渋谷 彰、小田 ちぐさ
  • Organizer: 第35回サイトプロテクション研究会
  • Place of Presentation: メルパルク京都（京都府京都市）
  • Invited
• [Presentation] バリア組織の細胞死とアレルギー、炎症疾患 (2017)
  • Author(s): 渋谷 彰
  • Organizer: Advanced Seminar Series on Microbiology and Immunology
  • Place of Presentation: 大阪大学微生物病研究所（大阪府吹田市）
  • Invited
• [Presentation] Clec10a, suppresses Der f-induced immune response and dermatitis (2016)
  • Author(s): Kanemaru K, Denda-Nagai K, Irimura T, Tahara-Hanaoka S, Shibuya A
  • Organizer: 第45回日本免疫学会総会・学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
• [Presentation] Characterization of mouse ES cell-derived mast cell line, MEDMC-BRC6 (2016)
  • Author(s): Shibagaki S, Tahara-Hanaoka S, Shibuya A
  • Organizer: 第45回日本免疫学会総会・学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
• [Presentation] Elovl6 attenuates mechanical stress-induced skin inflammation (2016)
  • Author(s): Nakamura Y, Nakahashi-Oda C, Shibuya A
  • Organizer: 第45回日本免疫学会総会・学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
• [Presentation] Involvement of DNAM-1 (CD226) expressed on small peritoneal macrophages in antigen presentation (2016)
  • Author(s): Vo AV, Takenaka E, Yamashita-Kanemaru Y, Shibuya A, Shibuya K
  • Organizer: 第45回日本免疫学会総会・学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
• [Presentation] Development of molecular targeted therapy for human acute GVHD in humanized mice (2016)
  • Author(s): Okumura G, Abe F, Ito M, Shibuya A, Shibuya K
  • Organizer: 第45回日本免疫学会総会・学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
• [Presentation] The regulatory role of Allergin-1 in autoantibody production (2016)
  • Author(s): Iizuka A, Segawa S, Kaneko S, Yokosawa M, Kondo Y, Tahara-Hanaoka S, Shibayama S, Goto D, Matsumoto I, Shibuya A, Sumida T
  • Organizer: 第45回日本免疫学会総会・学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
• [Presentation] Inhibitory Immunoreceptors in Inflammation and Allergy (2016)
  • Author(s): 渋谷 彰
  • Organizer: 京都大学医学研究科 免疫・アレルギー・感染コース リトリート
  • Place of Presentation: 白浜荘（滋賀県高島市）
  • Invited
• [Presentation] 死細胞によるアレルギー、炎症応答の制御 (2016)
  • Author(s): 渋谷 彰
  • Organizer: 千里ライフサイエンスセミナーK4 免疫・感染症シリーズ第6回「ワクチン、アジュバント開発研究の最前線」
  • Place of Presentation: 千里ライフサイエンスセンタービル（大阪府豊中市）
  • Invited
• [Presentation] Apoptotic epithelial cells control the abundance of regulatory T cell at barrier surfaces (2016)
  • Author(s): Nakazawa Y, Nakahashi-Oda C, Nakamura Y, Udayanga KGS, Shibuya A
  • Organizer: Immune Profiling in Health and Disease
  • Place of Presentation: The Fairmont Olympic Hotel, Seattle, WA, USA,
  • Int'l Joint Research
• [Presentation] 抑制性免疫受容体によるアレルギー炎症疾患の制御 (2016)
  • Author(s): 渋谷 彰
  • Organizer: Legend Seminar
  • Place of Presentation: 徳島大学 藤井節郎記念医科学センター（徳島県徳島市元町）
  • Invited
• [Presentation] 常在細菌叢によるバリア組織の恒常性維持 (2016)
  • Author(s): 渋谷 彰、小田 ちぐさ
  • Organizer: 第44回日本臨床免疫学会総会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Invited
• [Presentation] ヒト化マウスを用いた抗ヒトDNAM-1抗体によるヒト急性GVHDの治療法開発 (2016)
  • Author(s): 渋谷 和子、阿部 史枝、伊藤 守、渋谷 彰
  • Organizer: 第8回血液疾患免疫療法学会学術集会
  • Place of Presentation: 北海道大学医学部学友会館（北海道札幌市）
• [Presentation] Apoptotic epithelial cells control the abundance of regulatory T cells at barrier surfaces (2016)
  • Author(s): Nakahashi-Oda C, Udayanga K. G. S, Nakamura Y, Nakazawa Y, Totsuka N, Miki H, Iino S, Tahara-Hanaoka S, Honda S, Shibuya K, Shibuya A
  • Organizer: International Congress of Immunology 2016
  • Place of Presentation: Melbourne Convention and Ehibition Centre, Australia,
  • Int'l Joint Research
• [Presentation] CD155 (PVR/Necl5) mediates a costimulatory signal in CD4+ T cells and regulates allergic inflammation (2016)
  • Author(s): Yamashita-Kanemaru Y, Bernhardt G, Shibuya A, Shibuya K.
  • Organizer: International Congress of Immunology 2016
  • Place of Presentation: Melbourne Convention and Ehibition Centre, Australia
  • Int'l Joint Research
• [Presentation] Identification and characterization of CD300H, a new member of the Human CD300 immunoreceptor family (2016)
  • Author(s): Niizuma K, Tahara-Hanaoka S, Noguchi E, Shibuya A
  • Organizer: International Congress of Immunology 2016
  • Place of Presentation: Melbourne Convention and Ehibition Centre, Australia
  • Int'l Joint Research
• [Presentation] 抑制性免疫受容体による疾患制御 (2016)
  • Author(s): 渋谷 彰
  • Organizer: タイムシグナルと制御シンポジウム
  • Place of Presentation: 下田東急ホテル（静岡県下田市）
• [Presentation] 抗生物質による腸内細菌バランスの乱れと喘息 (2016)
  • Author(s): 渋谷 彰
  • Organizer: 第63回日本実験動物学会
  • Place of Presentation: ミューザ川崎シンフォニーホール（神奈川県川崎市）
  • Invited
• [Remarks] 免疫学研究室ホームページ
  • URL: http://immuno-tsukuba.com/index.html
• [Remarks] TRIOS
  • URL: http://trios.tsukuba.ac.jp/researcher/0000001643
• [Remarks] つくばリポジトリ
  • URL: https://tsukuba.repo.nii.ac.jp/index.php?action=pages_view_main&active_action=repository_view_main_item_snippet&index_id=543&pn=1&count=20&order=7&lang=japanese&page_id=13&block_id=83
• [Patent(Industrial Property Rights)] 抗ヒトＤＮＡＭ－１モノクローナル抗体及びその使用 (2016)
  • Inventor(s): 渋谷 彰、渋谷 和子、阿部 史枝、広近 玲、奥村 元紀
  • Industrial Property Rights Holder: 渋谷 彰、渋谷 和子、阿部 史枝、広近 玲、奥村 元紀
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-04-20