| Project/Area Number |
16K15552
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Psychiatric science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Ohmura Yu 北海道大学, 医学研究科, 助教 (80597659)
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| Co-Investigator(Kenkyū-buntansha) |
吉田 隆行 北海道大学, 医学研究院, 助教 (60374229)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMANAKA Akihiro 名古屋大学, 環境医学研究所, 教授 (60323292)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ゲノム編集 / セロトニン / 体温 / 5-HT1A受容体 / 精神薬理学 / 神経科学 |
|---|
| Outline of Final Research Achievements |
Because there are 14 subtypes of 5-HT receptor, conventional gene manipulations will require us to pay too much cost for examining all the subtypes. The purpose of this study was to establish a low-cost method for knocking out 5-HT receptor genes by injecting virus vector to the brain of Cas9-expressing mice. The hypothermic effect of serotonin 5-HT1A receptor agonist was attenuated in virus-injected mice. Analysis using DNA mismatch cleavage enzyme confirmed mutation of 5-HT1A receptor gene. However, we failed to obtain electrophysiological evidence using patch clamp methods because the condition of cells was not good.
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