Analysis of mesolimbic dopaminergic neuron (A10) induced from patients of mental disorders

Research Project

Project/Area Number	16K15561
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Psychiatric science
Research Institution	Kobe University
Principal Investigator	Sora Ichiro 神戸大学, 医学研究科, 教授 (40322713)
Co-Investigator(Kenkyū-buntansha)	毛利健太朗神戸大学, 保健管理センター, 助教 (00642125) 菱本明豊神戸大学, 医学研究科, 准教授 (50529526) 朴秀賢神戸大学, 医学研究科, 講師 (60455665)
Project Period (FY)	2016-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000) Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	Otx2 / Sox6 / 精神疾患 / 脳神経疾患 / 発生・分化
Outline of Final Research Achievements	Factors related to the differentiation and induction of the nigrostriatal dopamine neuron (A9) and the mesolimbic dopamine neuron (A10) were analized with SDIA（Stromal cell Derived Inducing Activity）method. As a result of examining differentiation and induction to dopaminergic neurons with SDIA method in various periods, it was found that Otx 2 and Sox 6 are expressed in about 3 weeks. Cells in which Otx 2 and Sox 6 are overlappingly expressed were not observed. Culture with dopamine neurons by SDIA method was thought to differentiate into and induce the nigrostriatal dopamine neuron (A9) and the mesolimbic dopamine neuron (A10) in about 3 weeks.