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Early diagnosis of colon cancer metastasis by multifaceted proteome analysis of metastatic lesion tissue and exosome

Research Project

Project/Area Number 16K15626
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionNational Institutes of Biomedical Innovation, Health and Nutrition

Principal Investigator

Tomonaga Takeshi  国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 プロテオームリサーチプロジェクト, プロジェクトリーダー (80227644)

Co-Investigator(Renkei-kenkyūsha) NAGAYAMA Satoshi  公益財団法人がん研究会, 有明病院消化器外科, 医長 (70362499)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords大腸がん / 転移早期診断 / エクソソーム / プロテオミクス / 小腸大腸肛門外科学 / 再発早期診断
Outline of Final Research Achievements

In this study, we aimed to explore the metastatic marker from colorectal cancer tissue and to develop an early diagnosis method of metastasis using blood exosome. Quantitative proteomic analysis of mambrane proteins in 24 tissues of primary and metastatic lesion in the same patient with colorectal cancer identified 182 potential metastatic biomarker candidate proteins with different expression levels between primary and metastatic lesion . Next, we examined whether these proteins can be detected and quantified in blood exosomes using the SRM / MRM method. As a result, it was suggested that two proteins could diagnose metastatic colorectal cancer with high accuracy. These proteins were considered promising as a metastatic marker of colorectal cancer.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (17 results)

All 2018 2017 2016 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 4 results,  Invited: 9 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics2017

    • Author(s)
      Shiromizu Takashi、Kume Hideaki、Ishida Mimiko、Adachi Jun、Kano Masayuki、Matsubara Hisahiro、Tomonaga Takeshi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 12782-12782

    • DOI

      10.1038/s41598-017-13092-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proteomic analysis of urinary extracellular vesicles from high Gleason score prostate cancer.2017

    • Author(s)
      Fujita K, Kume H, Matsuzaki K, Kawashima A, Ujike T, Nagahara A, Uemura M, Miyagawa Y, Tomonaga T, Nonomura N.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 42961-42961

    • DOI

      10.1038/srep42961

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Improved Proteome and Phosphoproteome Analysis on a Cation Exchanger by a Combined Acid and Salt Gradient.2016

    • Author(s)
      Adachi, J., Hashiguchi, K., Nagano, M., Sato, M., Sato, A., Fukamizu, K., Ishihama, Y., Tomonaga, T
    • Journal Title

      Anal Chem

      Volume: 88 Issue: 16 Pages: 7899-7903

    • DOI

      10.1021/acs.analchem.6b01232

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Discovery of colorectal cancer biomarker in serum extracellular vesicles by targeted proteomics.2018

    • Author(s)
      Takeshi Tomonaga
    • Organizer
      US-Japan Workshop for Cancer Research
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 大腸がんの早期診断・再発診断バイオマーカーの探索2017

    • Author(s)
      朝長 毅
    • Organizer
      日本臨床検査医学会・日本臨床化学会 第13回合同地方会
    • Place of Presentation
      岡山
    • Year and Date
      2017-02-12
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 先端プロテオーム解析技術を用いた新規創薬標的因子の探索2017

    • Author(s)
      朝長 毅
    • Organizer
      彩都産学官連携フォーラム2017
    • Place of Presentation
      大阪
    • Year and Date
      2017-01-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] ターゲットプロテオミクスを用いた血清エクソソーム中の大腸がん早期診断マーカー開発と臨床応用.2017

    • Author(s)
      朝長 毅、白水 崇、足立 淳
    • Organizer
      第65回質量分析総合討論会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics.2017

    • Author(s)
      Takeshi Tomonaga, Takashi Shiromizu, Jun Adachi
    • Organizer
      65TH ASMS Conference on Mass Spectrometry and Allied Topics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics.2017

    • Author(s)
      Takeshi Tomonaga, Takashi Shiromizu, Jun Adachi
    • Organizer
      16th Human Proteome Organization World Congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 臨床検体を用いた創薬標的探索のためのプロテオーム・リン酸化プロテオーム解析~分離と選択の融合~2017

    • Author(s)
      朝長 毅
    • Organizer
      生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 質量分析計を用いたクリニカルプロテオミクスの可能性を探る2016

    • Author(s)
      朝長 毅
    • Organizer
      第36回日本分子腫瘍マーカー研究会
    • Place of Presentation
      横浜
    • Year and Date
      2016-10-05
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Verification of colorectal cancer biomarker candidates in plasma/serum extracellular vesicles by targeted proteomics.2016

    • Author(s)
      Takeshi Tomonaga
    • Organizer
      15th Human Proteome Organization World Congress
    • Place of Presentation
      台北
    • Year and Date
      2016-09-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 質量分析計を用いたクリニカルプロテオミクスの可能性を探る2016

    • Author(s)
      朝長 毅
    • Organizer
      質量分析により変貌する臨床検査の最前線2016
    • Place of Presentation
      東京
    • Year and Date
      2016-09-12
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 定量プロテオミクスを用いたバイオマーカー探索2016

    • Author(s)
      朝長 毅、白水 崇、足立 淳
    • Organizer
      第 29 回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2016-09-02
    • Related Report
      2016 Research-status Report
    • Invited
  • [Remarks] 国立研究開発法人 医薬基盤・健康・栄養研究所プロテオームリサーチプロジェクト

    • URL

      http://www.nibiohn.go.jp/proteome/

    • Related Report
      2017 Annual Research Report
  • [Remarks] http://www.nibiohn.go.jp/proteome/

    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 大腸がんを検出するためのバイオマーカー2017

    • Inventor(s)
      朝長 毅、白水 崇
    • Industrial Property Rights Holder
      デンカ生研株式会社、国立研究開発法人 医薬基盤・健康・栄養研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-129941
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2019-03-29  

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