| Project/Area Number |
16K15667
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
宿南 知佐 広島大学, 医歯薬保健学研究科(歯), 教授 (60303905)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 腱 / 靭帯 / microRNA / Dicer / Scleraxis / 発生・成熟 / 組織修復 / 腱・靭帯 / 組織再生・修復 / 再生 |
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| Outline of Final Research Achievements |
The purpose of this study is to examine the role of microRNA (miRNA) in the development and maturation of tendon/ligament using Scleraxis-Cre knock-in:Dicer flox mice. We performed miRNA expression profiling using achilles tendon tissues and Achilles tendon-derived cells. Several miRNAs highly expressed in Achilles tendon. To examine the role of miRNAs in development and maturation of tendon/ligament, we generated Scleraxis-Cre knock-in:Dicer flox mice. Dicer is one of the most important enzyme in miRNA biogenesis. Scleraxis-Cre knock-in:Dicer flox mice showed impaired tendon maturation and healing. Highly expressed miRNAs in tendon were decreased in achilles tendon of these mice. Thus, these data suggest that miRNA play an important role in maturation and healing of tendon/ligament.
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