analysis in function of androgen in testcular germ cell tumor

• Project/Area Number: 16K15695
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Ueda Takashi 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (50601598)
• Co-Investigator(Kenkyū-buntansha): 中村 晃和 京都府立医科大学, 医学(系)研究科(研究院), 客員教授 (10381964) ; 浮村 理 京都府立医科大学, 医学(系)研究科(研究院), 教授 (70275220) ; 大石 正勝 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (90405316) ; 上田 紗弥 (伊藤紗弥) 京都府立医科大学, 医学(系)研究科(研究院), 博士研究員 (90534511) ; 本郷 文弥 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (80291798) ; 金沢 元洪 京都府立医科大学, 医学(系)研究科(研究院), 助教 (00468266) ; 藤原 敦子 京都府立医科大学, 医学(系)研究科(研究院), 講師 (20457980)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アンドロゲン / 精巣腫瘍 / セミノーマ / TPH1 / セロトニン / AR / アンドロゲンレセプター

Outline of Final Research Achievements

Epidemiological findings suggest that andorogen/AR signal has a role in semioma progression. However, the role of androgen/AR signal in seminoma progression remains unclear.
Additon of androgen in seminoma cell line (TCam-2) suppressed cell growth and suppression of androgen/AR signal in xenograft model mouse promoted cell growth of TCam-2.Activation of androgen/AR signaling in TCam-2 cells reduced the expression of TPH1 in SE cells, followed by the reduction of serotonin secretion in cell culture supernatant. These results suggested that silencing of androgen/AR signaling may cause initiation and progression of seminoma through increase in TPH1 gene expression level.

Research Products

• [Journal Article] CNPY2 promoted the proliferation of renal cell carcinoma cells and increased the expression of TP53. (2017)
  • Author(s): Taniguchi H, Ito S, Ueda T, Morioka Y, Kayukawa N, Ueno A, Nakagawa H, Fujihara A, Ushijima S, Kanazawa M, Hongo F, Ukimura O.
  • Journal Title: Biochem Biophys Res Commun. Volume: 485 Issue: 2 Pages: 267-271
  • DOI: 10.1016/j.bbrc.2017.02.095
  • Peer Reviewed / Open Access
• [Journal Article] Androgen suppresses testicular cancer cell growth in vitro and in vivo. (2016)
  • Author(s): Nakagawa H, Ueda T, Ito S, Shiraishi T, Taniguchi H, Kayukawa N, Nakanishi H, Ushijima S, Kanazawa M, Nakamura T, Naya Y, Hongo F, Kamoi K, Okihara K, Ukimura O
  • Journal Title: Oncotarget Volume: 7 Issue: 23 Pages: 35224-32
  • DOI: 10.18632/oncotarget.9109
  • Peer Reviewed / Open Access
• [Presentation] analysis in function of androgen in seminoma (2018)
  • Author(s): 上田崇
  • Organizer: 日本泌尿器科学会
• [Presentation] アンドロゲンによる精巣腫瘍増殖抑制効果の解析 (2017)
  • Author(s): 中河秀生
  • Organizer: 泌尿器分子細胞研究会
• [Presentation] アンドロゲンによる精巣腫瘍増殖抑制効果の解析 (2016)
  • Author(s): 中河秀生
  • Organizer: 日本癌学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-07