| Project/Area Number |
16K15728
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Hokkaido University |
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Principal Investigator |
Ishida Susumu 北海道大学, 医学研究院, 教授 (10245558)
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| Co-Investigator(Kenkyū-buntansha) |
西村 紳一郎 北海道大学, 先端生命科学研究院, 教授 (00183898)
野田 航介 北海道大学, 医学研究院, 准教授 (90296666)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 血管内皮増殖因子受容体-2 / N型糖鎖 / 糖尿病網膜症 / 低酸素 / 糖鎖 / 眼細胞生物学 |
|---|
| Outline of Final Research Achievements |
Vascular endothelial growth factor (VEGF), a potent angiogenic and vascular permeability factor, plays a significant role in the pathogenesis of diabetic retinopathy. In the current study, we analyzed the structures of N-glycans on the receptor of VEGF, VEGFR-2. On the basis of preexisting database for glycan structure, the 16 structures of N-glycans on VEGFR-2 were identified in human retinal microvascular endothelial cells (HRMECs). However, no alteration of N-glycans was found on VEGFR-2 in HRMECs cultured under hypoxic condition, one of the representative pathological condition in diabetic retinopathy, for 24 hours compared to that under normoxic condition.
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