| Project/Area Number |
16K15770
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Osaka Institute of Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
丸山 征郎 鹿児島大学, 医歯学総合研究科, 特任教授 (20082282)
升田 好樹 札幌医科大学, 医学部, 教授 (10244328)
三浦 直樹 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (80508036)
伊藤 隆史 鹿児島大学, 医学部・歯学部附属病院, その他 (20381171)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 敗血症 / アラーミン / ヌクレオフォスミン / DAMPs |
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| Outline of Final Research Achievements |
Nucleophosmin (NPM), which is a nucleoprotein, is major multifunctional protein involved in ribosomal biogenesis, centrosome duplication, cell cycle progression, apoptosis, and cell differentiation. Recently, NPM is released from endotoxin-stimulated macrophages and induces inflammatory cytokines such as tumor necrosis factor-alpha and interleukin-6, suggesting that NPM might be an alarmin. In this study, to administer NPM to mouse, we established vectors of full-length NPM and found that the recombinant (r) NPM induced TNF-alpha in mouse macrophage-like RAW264.7 cells. In the next step, we are going to administer rNPM to mouse.
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