Search of master regulator of odontoblastogenesis using iPS interference

• Project/Area Number: 16K15802
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Okayama University
• Principal Investigator: Ono Mitsuaki 岡山大学, 医歯薬学総合研究科, 助教 (60613156)
• Co-Investigator(Kenkyū-buntansha): 窪木 拓男 岡山大学, 医歯薬学総合研究科, 教授 (00225195) ; 大島 正充 徳島大学, 大学院医歯薬学研究部(歯学系), 准教授 (00548307) ; 前川 賢治 岡山大学, 医歯薬学総合研究科, 准教授 (20304313) ; 大橋 俊孝 岡山大学, 医歯薬学総合研究科, 教授 (50194262) ; 渡辺 亮 京都大学, iPS細胞研究所, 特定拠点助教 (60506765)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 象牙芽細胞分化 / マスター遺伝子 / 象牙芽細胞 / iPS細胞 / プログラミング

Outline of Final Research Achievements

It is still unknown not only the mechanism but also the master regulator of odontoblastogenesis. Therefore, the final goal of our study is to identify the mater regulator of odontoblastogenesis. We performed the comprehensive analysis from the histological and developmental point of view using RNA-Seq, and could narrow down the several candidate odontoblastogenesis related genes. In the future, we are planing to perform the functional analysis of these candidate genes.

Research Products

• [Journal Article] Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium (2018)
  • Author(s): Komori T, Ono M, Hara ES, Ueda J, Nguyen HTT, Nguyen HT, Yonezawa T, Maeba T, Ono A, Takarada T, Momota R, Maekawa K, Kuboki T, Oohashi T
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 2612-2612
  • DOI: 10.1038/s41598-018-21000-0
  • Peer Reviewed / Open Access
• [Journal Article] CCN4/WISP1 controls cutaneous wound healing by modulating proliferation, migration and ECM expression in dermal fibroblasts via α5β1 and TNFα. (2018)
  • Author(s): Ono M, Masaki A, Maeda A, Kilts TM, Hara ES, Komori T, Pham H, Kuboki T, Young MF.
  • Journal Title: Matrix Biol. Volume: 17 Pages: 30400-6
  • DOI: 10.1016/j.matbio.2018.01.004
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ammonium tetrathiomolybdate enhances the antitumor effects of cetuximab via the suppression of osteoclastogenesis in head and neck squamous carcinoma. (2018)
  • Author(s): Morisawa A, Okui T, Shimo T, Ibaragi S, Okusha Y, Ono M, Nguyen TTH, Hassan NMM, Sasaki A.
  • Journal Title: International Journal of Oncology Volume: 52 Pages: 989-999
  • DOI: 10.3892/ijo.2018.4242
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Physiological role of urothelial cancer-associated one long noncoding RNA in human skeletogenic cell differentiation (2017)
  • Author(s): Ishikawa T, Nishida T, Ono M, Takarada T, Nguyen HT, Kurihara S, Furumatsu T, Murase Y, Takigawa M, Oohashi T, Kamioka H, Kubota S
  • Journal Title: J Cell. Physiol. Volume: 233(6) Issue: 6 Pages: 4825-4840
  • DOI: 10.1002/jcp.26285
  • Peer Reviewed / Open Access
• [Presentation] 必須アミノ酸Tryptophanは骨髄由来間葉系幹細胞の幹細胞性を制御し骨形成を促進する． (2017)
  • Author(s): 大野充昭，Pham HT, 笈田育尚，小盛大志，土佐郁恵，大橋俊孝，秋山謙太郎，窪木拓男．
  • Organizer: 第47回公益社団法人日本口腔インプラント学会学術大会
• [Presentation] Regulation of gingival keratinization by laminin332. (2017)
  • Author(s): Komori T, Ueda J, Ono M, Sonoyama W, Hara ES, Yoshioka Y, Maekawa K, Kuboki T．
  • Organizer: 95th General Session & Exhibition of the IADR.
  • Int'l Joint Research
• [Presentation] BMP antagonist inhibitor L51P regulates the chondrogenesis and osteoarthritis. (2017)
  • Author(s): Nguyen HT, Ono M, Komori T, Oohashi T, Kuboki T.
  • Organizer: The 65th Annual Meeting of JADR.
  • Int'l Joint Research
• [Presentation] イヌ自家移植モデルにおける器官原基法を応用した歯の再生の試み． (2017)
  • Author(s): 大野充昭，大島正充， 園山 亘，小川美帆，笈田育尚，Emilio HS，新川重彦，中島 隆，辻 孝，窪木拓男．
  • Organizer: 第3回日本骨免疫学会．
• [Presentation] ヒト骨髄由来間葉系幹細胞および軟骨細胞の核内の染色体配置の解析の試み． (2017)
  • Author(s): 大野充昭，小盛大志，土佐郁恵，秋山謙太郎，大野 彩，窪木拓男，大橋俊孝．
  • Organizer: 公益社団法人日本補綴歯科学会第126回学術大会．