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Establishment of a novel and easy in vitro toxicity evaluation system using freezed cell spheroid

Research Project

Project/Area Number 16K15815
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Dental engineering/Regenerative dentistry
Research InstitutionMatsumoto Dental University

Principal Investigator

Kagami Hideaki  松本歯科大学, 総合歯科医学研究所, 教授 (80242866)

Co-Investigator(Kenkyū-buntansha) 李 憲起  松本歯科大学, 歯学部, 講師 (60350831)
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsin vitro試験 / 細胞毒性 / 線維芽細胞 / 凍結保存 / 動物実験 / 凍結方法 / 代償技術 / 細胞培養 / 動物実験代償技術 / 安全性試験 / 細胞・組織 / 生理活性 / 薬剤反応性
Outline of Final Research Achievements

The purpose of this study was to develop a in vitro test for cell toxicity using a novel cell freezing technology. Mouse skin-derived fibroblasts were used. The cells cultured on a regular dish could not survive, On the other hand, the spheroids from fibroblasts could survive and proliferate after freeze-thaw cycle. Then, various freezing conditions including spheroid size, the concentration of cryoprotectant, and the volume of cryoprotectact were optimized. From this study, we could successfully achieved the in vitro cell toxicity test system using spheroids, which can be used immediately after thaw with culture medium without washing process.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (16 results)

All 2018 2017 2016

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Issues with the surgical treatment of antiresorptive agent-related osteonecrosis of the jaws.2018

    • Author(s)
      Kagami H, Kobayashi A, Taguchi A, Li X, and Yoshizawa M
    • Journal Title

      Oral Dis.

      Volume: 24 Issue: 1-2 Pages: 52-56

    • DOI

      10.1111/odi.12783

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential application of tissue engineering for the reconstruction of facial bones.2017

    • Author(s)
      Kagami H
    • Journal Title

      Oral Dis.

      Volume: 23 Issue: 6 Pages: 689-691

    • DOI

      10.1111/odi.12581

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Adipocytes enhance the expression of osteoclast adhesion-related molecules through CXCL12/CXCR4 signaling pathway2016

    • Author(s)
      Luo T, Liu H, Feng W, Liu D, Du J, Sun J, Wang W, Han X, Guo J, Amizuka N, Li X, Li M.
    • Journal Title

      Cell Prolif

      Volume: - Issue: 3

    • DOI

      10.1111/cpr.12317

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Discontinuation of simvastatin lead to a rebound phenomenon and result in immediate peri-implant bone loss2016

    • Author(s)
      Li X, Wu F, Zhang Y, Yang J, Shinohara A, Kagami H
    • Journal Title

      Clinical and Experimental Dental Research

      Volume: 2(1) Issue: 1 Pages: 65-72

    • DOI

      10.1002/cre2.23

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Effect of cell seeding conditions on the efficiency of in vivo bone formation.2016

    • Author(s)
      Hori A, Agata H, Takaoka M, Tojo A, Kagami H
    • Journal Title

      Int J Oral Maxillofac Implants

      Volume: 31 Issue: 1 Pages: 232-239

    • DOI

      10.11607/jomi.4729

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 根管重鎮剤の逸脱に起因した上顎洞炎の1症例2016

    • Author(s)
      小林明人、田口明、篠原淳、各務秀明
    • Journal Title

      松本歯学

      Volume: 42 Pages: 25-31

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 上顎洞に広範囲に進展したKeratocystic Odontogenic Tumorの1例2016

    • Author(s)
      山田真一郎、内田啓一、高田匡基、嶋田勝光、落合隆永、杉野紀幸、長谷川博雅、各務秀明、田口明
    • Journal Title

      松本歯学

      Volume: 42 Pages: 111-117

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 左側頬部に発生した筋肉内脂肪腫の1例2016

    • Author(s)
      内田啓一、落合隆永、斉藤安奈、杉野紀幸、中野敬介、長谷川博雅、芳澤享子、各務秀明、田口明
    • Journal Title

      松本歯学

      Volume: 42 Pages: 104-110

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Effect of cell processing protocol on the clinical result of bone tissue engineering2017

    • Author(s)
      Kagami H, Inoue M, Li X, Nagamura-Inoue T, Tojo A, Yamashita N
    • Organizer
      Translational Opportunities in Stem Cell Research
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 大学病院における閉鎖型自動細胞培養装置を用いた細胞培養とその経過2017

    • Author(s)
      各務秀明,李憲起,秋山裕和,市村昌紀,宇田川信之
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 自動培養装置と簡易型クリーンブースを用いた特定細胞加工物調製システムの構築2017

    • Author(s)
      李 憲起, 芳澤享子, 各務秀明
    • Organizer
      第62回(公社)日本口腔外科学会総会・学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 歯槽骨再生治療の実現のための細胞調製システムの構築とその運用「大学病院における閉鎖型自動細胞培養装置を用いた細胞培養とその経過」2017

    • Author(s)
      各務秀明
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] T2緩和差を利用した31P-NMRによる非侵襲的な新生骨量・骨量測定法の開発2016

    • Author(s)
      斉藤安奈、八上公利、高田匡基、井上 実、 森こず恵、 李 憲起、田口 明、各務秀明、芳澤享子 、篠原 淳
    • Organizer
      第61回(公社)日本口腔外科学会 総会・学術大会
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Research-status Report
  • [Presentation] 骨再生治療:基礎研究から臨床応用まで骨髄間質細胞を用いた骨再生治療2016

    • Author(s)
      各務秀明, 井上実, 朝比奈泉, 宇田川信之.
    • Organizer
      第23回歯科医学会総会 シンポジウム
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-10-21
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Somatic stem cells and their application for bone tissue engineering .2016

    • Author(s)
      Kagami H.
    • Organizer
      Tenth People’s Hospital Tongji University Invited Lecture.
    • Place of Presentation
      Shanghai (China)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Book] 末長書店2016

    • Author(s)
      各務秀明、井上実、長谷川博雅(山根源之他編)
    • Total Pages
      640
    • Publisher
      口腔内科学
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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