Development of a microRNA measurement system based on a multiplexed fluorescence readout technique

• Project/Area Number: 16K16408
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical systems
• Research Institution: Osaka University
• Principal Investigator: Takahiro Nishimura 大阪大学, 工学研究科, 助教 (10722829)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 光情報処理 / 蛍光計測 / 生体分子計測 / 簡易診断 / 分子情報処理 / バイオマーカー / DNAナノエンジニアリング / バイオセンシング / 蛍光通信 / ポイントオブケア / DNAナノテクノロジー / POCT / 蛍光 / 光通信

Outline of Final Research Achievements

This study aims to develop a multiplexed fluorescence readout method using time varied fluorescence signals for point of care testing of microRNA (miRNA) biomarkers. Multiplexed fluorescence readout was experimentally confirmed with fluorescence coded DNA strands and three molecular beacons. Numerical simulation demonstrated that the time-varied fluorescence signals can enhance the readout accuracy.

Academic Significance and Societal Importance of the Research Achievements

本研究の特色として、光・分子情報処理をmiRNA検出へ応用することにより、情報通信技術のナノ領域への実用展開の一例を与えることが挙げられる。分子検出において、分子レベルでの情報加工と蛍光読み出しへ高度な情報通信技術を適用できれば、物質により制限された条件において最大限に情報を伝送することができる。本研究の成果は、安価で簡単な操作によるmiRNAバイオマーカー検出を可能にし、癌の超早期発見や進行ステージの的確かつ迅速な診断につながる。これは、医療現場に留まらず、日常生活における健康状態の把握を高精度で可能にし、未病段階での予防や早期治療の普及につながることが期待できる。

Research Products

• [Journal Article] Experimental assessment and analysis of super-resolution in fluorescence microscopy based on multiple-point spread function fitting of spectrally demultiplexed images (2018)
  • Author(s): Nishimura Takahiro、Kimura Hitoshi、Ogura Yusuke、Tanida Jun
  • Journal Title: Optical Review Volume: 25 Issue: 3 Pages: 384-390
  • DOI: 10.1007/s10043-017-0379-y
  • Peer Reviewed
• [Journal Article] Fluorescence encoded super resolution imaging based on a location estimation algorithm for high-density fluorescence probes (2017)
  • Author(s): T. Nishimura, H. Kimura, Y. Ogura, and J. Tanida
  • Journal Title: Optical Review Volume: 24 Issue: 2 Pages: 212-218
  • DOI: 10.1007/s10043-016-0286-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Multiplexed fluorescence readout using time responses of color coded signals for biomolecular detection (2016)
  • Author(s): T. Nishimura, Y. Ogura, and J. Tanida
  • Journal Title: Biomedical Optics Express Volume: 7 Issue: 12 Pages: 5284-5293
  • DOI: 10.1364/boe.7.005284
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Optically controlled release of DNA based on nonradiative relaxation process of quenchers (2016)
  • Author(s): Y. Ogura, A. Onishi, T. Nishimura, and Jun Tanida
  • Journal Title: Biomedical Optics Express Volume: 7 Issue: 6 Pages: 2142-2153
  • DOI: 10.1364/boe.7.002142
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Lateral spatial resolution improvement in laser scanning fluorescence microscopy using a subdiffraction limit optical spot (2018)
  • Author(s): Takahiro Nishimura, Daiki Shinkawa, Yusuke Ogura, Yosuke Tamada, Jun Tanida
  • Organizer: Biomedical Imaging and Sensing Conference
  • Int'l Joint Research
• [Presentation] Fluorescence imaging of photosensitizers in biological tissues for photodynamic diagnosis during interstitial photodynamic therapy (2018)
  • Author(s): Takahiro Nishimura, Kunio Awazu
  • Organizer: Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXVIII
  • Int'l Joint Research
• [Presentation] Computational simulation of the effect of interstitial Photodynamic therapy for pretreatment planning (2018)
  • Author(s): Takahiro Nishimura
  • Organizer: The 8th Japan-Korea Digital Holography and Information Processing (DHIP)
  • Int'l Joint Research / Invited
• [Presentation] A molecular-sized optical logic circuit for digital modulation of a fluorescence signal (2017)
  • Author(s): Nishimura Takahiro、Ogura Yusuke、Tanida Jun
  • Organizer: Third International Conference on Photonics Solutions (ICPS2017)
  • Int'l Joint Research
• [Presentation] 波長符号とベイズ解析に基づく超解像蛍光イメージング (2017)
  • Author(s): 西村隆宏, 小倉裕介, 谷田純
  • Organizer: 一般社団法人レーザー学会学術講演会
  • Invited
• [Presentation] 蛍光符号化とベイズ情報量規準を用いた超解像蛍光イ メージングの実証実験 (2017)
  • Author(s): 木村仁, 西村隆宏, 小倉裕介, 谷田純
  • Organizer: 第64回応用物理学会春季学術講演会
  • Place of Presentation: 横浜
• [Presentation] Bayesian-based localization of fluorescence-encoded images for single shot super-resolution fluorescence imaging (2016)
  • Author(s): H. Kimura, T. Nishimura, Y. Ogura, and J. Tanida
  • Organizer: The 2nd Biomedical Imaging and Sensing Conference 2016 (BISC'16)
  • Place of Presentation: 横浜
  • Year and Date: 2016-05-18
  • Int'l Joint Research
• [Presentation] ベイズ情報量基準に基づく量子ドットを用いた蛍光符号化イメージングの基礎的実験 (2016)
  • Author(s): 木村仁, 西村隆宏, 小倉裕介, 谷田純
  • Organizer: Optics & Photonics Japan 2016
  • Place of Presentation: 東京