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System construction to identify the cause of cocaine sensitization using epigenetics analysis

Research Project

Project/Area Number 16K16648
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Basic / Social brain science
Research InstitutionKurume University

Principal Investigator

Ohnishi Yoko  久留米大学, 医学部, 助教 (70727586)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsうつ病 / 依存症 / 転写因子 / エピジェネティクス / 行動実験 / コカイン / AP-1 / プロテアソーム / マウス / プロテオソーム / 側坐核 / 薬物依存症
Outline of Final Research Achievements

We have reported overexpression of deltaFosB in nucleus accumbens worked as anti-depressants, enhanced cocaine addiction after stress, and elevated cocaine sensitization. We tried to find binding partners of deltaFosB by Yeast two hybrid methods, and found several candidates including c-Jun and JunD, which are already known binding partners. We focused on Psmc5 as deltaFosB binding partner, which is one of proteasome machinery. Psmc5 enhanced deltaFosB expression and was induced by cocaine administration. Overexpression of Psmc5 in nucleus accumbens enhanced cocaine sensitization, but deletion form of deltaFosB binding domain of Psmc5 didn’ t have such effects.

Academic Significance and Societal Importance of the Research Achievements

依存症やうつ病における脳内の分子的変化と行動に対する影響を明らかにすることで、生物学的に脳の順応反応のメカニズムを明らかにできるところが学術的に意義があります。さらに社会的には、アルコール中毒、たばこ中毒、薬物依存、うつ病といった病気の発症メカニズムを明らかにできることで今後の治療方針、治療薬の開発に役立つ。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2018 2017

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Generation and validation of a floxed FosB mouse line2017

    • Author(s)
      Ohnishi Yoshinori N.、Eagle Andrew L.、Ohnishi Yoko H.、Cahill Michael E.、Wirtz Alexis J.、Robison Alfred J.、Nestler Eric J.
    • Journal Title

      biorxiv

      Volume: 1 Pages: 1-1

    • DOI

      10.1101/179309

    • Related Report
      2017 Research-status Report
    • Open Access / Int'l Joint Research
  • [Presentation] Is it possible that non-attractive male mouse can get attractive mind set?2018

    • Author(s)
      Ohnishi YN, Kawahara Y, Ohnishi YH, Nishi A
    • Organizer
      Neuroscience 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] モテるオスにはどうしたらなれるのか?2018

    • Author(s)
      大西克典、河原幸江、大西陽子、西 昭徳
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 長期間の抗酸化食物摂取は社会的ストレス後のコカイン依存症を抑制する2017

    • Author(s)
      大西克典、河原幸江、大西陽子、Neve RL、Vialou VF、Nestler EJ、西 昭徳
    • Organizer
      第90回日本薬理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Is it possible that non-attractive male mouse could get female mind?2017

    • Author(s)
      Ohnishi YN, Kawahara Y, Ohnishi YH, Nishi A
    • Organizer
      Neuroscience 2017
    • Related Report
      2017 Research-status Report
  • [Presentation] モテモテ大作戦!女の子にモテる方法、モテなくなる方法2017

    • Author(s)
      大西克典、河原幸江、大西陽子、西 昭徳
    • Organizer
      第28回マイクロダイアリシス研究会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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