| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Outline of Final Research Achievements |
A new hyperspectral imaging spectrometer has been developed. The newly designed system is based on a conventional confocal microscope with additional two galvano-mirror scanner units, one for the excitation laser, and the other for the signal light. By driving the scanner units synchronously, simultaneous detection of signal light from multiple spots on the sample on a charge-coupled-device (CCD) detector has been realized. Hyperspectral imaging of cultured cells and bio-tissue was demonstrated to show fast (12 msec / spectrum) and high sensitive spectral acquisition capability of the system. Owing to its spectral information, localization of multiple biomolecules in a sample was visualized simultaneously with the spatial resolution of the images comparable to that obtained by conventional confocal microscopes.
|
