• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of molecular mechanism of complex brain by ferret

Research Project

Project/Area Number 16K18382
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nerve anatomy/Neuropathology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Yuji Tsunekawa  国立研究開発法人理化学研究所, 多細胞システム形成研究センター, 研究員 (80733352)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords複雑脳発生 / de novo Cre KI / 複雑脳におけるAscl1の機能解析 / de novoノックイン技術の開発 / 複雑脳の発生
Outline of Final Research Achievements

The purpose of this project is to understand molecular mechanism of development of the complex gyrencephalic brain. Mice, which is most used model animals to study neural development but they dont't have gyruses in their brain. Because of this fact, I used the ferret as the model animal of this study. Ferret is a samll handy animals which have gyrencephalic brain, however, it is still difficult to apply genome editing technology to ferret. To solve this problem and progress this project, I reported novel de novo targeted gene knock-in techniques mediated by in utero electroporation and Adeno-associated virus (AAV) into the developing and developed complex brain. Further study to understand molecular mechanism of development of the complex gyrencephalic brain will be required by these newly developed technology.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Developing a De Novo Targeted Knock-in Method Based on in Utero Electroporation Into the Mammalian Brain2016

    • Author(s)
      Tsunekawa, Yuji, Raymond Kunikane Terhune, Ikumi Fujita, Atsunori Shitamukai, Taeko Suetsugu, Fumio Matsuzaki
    • Journal Title

      Development

      Volume: 143 Issue: 17 Pages: 3216-3222

    • DOI

      10.1242/dev.136325

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration.2016

    • Author(s)
      Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X, ---, Belmonte JC.
    • Journal Title

      Nature

      Volume: 540(7631) Issue: 7631 Pages: 144-149

    • DOI

      10.1038/nature20565

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Developing de novo genome editing meyhods2017

    • Author(s)
      恒川雄二
    • Organizer
      Conbio2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Patent(Industrial Property Rights)] US provisional application 62/363,1642016

    • Inventor(s)
      Yuji Tsunekawa
    • Industrial Property Rights Holder
      Yuji Tsunekawa
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-07-15
    • Related Report
      2016 Research-status Report
    • Overseas

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi