Functional analysis of KCTD protein as a novel angiogenic regulator
| Project/Area Number |
16K18421
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Ehime University |
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Principal Investigator |
Sakaue Tomohisa 愛媛大学, 医学系研究科, 助教(特定教員) (20709266)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 血管新生 / CUL3 / ユビキチン / 細胞骨格 / アクチン / 細胞運動 / Rho / 血管内皮細胞 / KCTD / アクチン重合 |
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| Outline of Final Research Achievements |
We have recently found CUL3-KCTD complex, one of the E3 ubiquitin ligase, as a novel angiogenic factor. In this study, we screened the substrate of CUL3-KCTD complex using wheat cell-free synthesized protein array, resulting in the successful identification of regulator of GTP-RhoA. Knockdown of CUL3 or KCTD led to severe anti-angiogenic phenotypes due to accumulation of the substrate in endothelial cells. These data suggested that CUL3-KCTD would tightly regulate the substrate protein which might play a crucial role for vascular elongation during angiogenesis.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] The CUL3-SPOP-DAXX axis is a novel regulator of VEGFR2 expression in vascular endothelial cells2017
Author(s)
Sakaue T, Sakakibara I, Fujisaki A, Uesugi T, Nakashiro K, Hamakawa H, Kubota E, Joh T, Imai Y, Izutani H, Higashiyama S.
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Journal Title
Sci Rep.
Volume: 7
Issue: 1
Pages: 42845-42845
DOI
Related Report
Peer Reviewed / Open Access
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