Establishment of monitoring method for Ad-REIC-based therapeutic effects on cancer using the newly developed anti-REIC antibody that recognizes the cancer specific REIC conformation
| Project/Area Number |
16K18440
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnostics
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| Research Institution | Okayama University |
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Principal Investigator |
Kinoshita Rie 岡山大学, 医歯薬学総合研究科, 助教 (40518297)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | REIC / 遺伝子治療 / がん / がん抑制遺伝子 / モノクローナル抗体 / ELISA / 癌 / 蛋白質 |
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| Outline of Final Research Achievements |
Reduced expression in immortalized cells (REIC) is a tumor suppressor gene and has been studied as a promising therapeutic gene for cancer gene therapy. Intratumoral injection of adenovirus vector carrying the human REIC (Ad-REIC) elicits cancer cell-specific apoptosis and anti-cancer immune responses. In addition, Ad-REIC gene therapy resulted in complete response in the case of metastatic castration-resistant prostate cancer patient. We obtained various anti-REIC monoclonal antibodies and developed a sandwich ELISA method for measuring the REIC protein concentration in the human blood. Using this assay, we have successfully developed the method for monitoring cancer treatment with Ad-REIC.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Expression of tumor suppressor REIC/Dkk-3 by a newly improved adenovirus vector with insertion of an hTERT promoter at the 3’-side of the transgene.2017
Author(s)
Putranto EW, Kinoshita R, Watanabe M, Sadahira T, Murata H, Yamamoto KI, Futami J, Kataoka K, Inoue Y, Ruma IM, Sumardika IW, Youyi C, Kubo M, Sakaguchi Y, Saito K, Nasu Y, Kumon H, Huh NH, and Sakaguchi M.
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Journal Title
Oncol. Lett.
Volume: 14
Issue: 1
Pages: 1041-1048
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Robust cancer-specific gene expression by a novel cassette with hTERT and CMV promoter elements.2017
Author(s)
Sakaguchi M, Sadahira T, Ueki H, Kinoshita R, Murata H, Yamamoto KI, Futami J, Nasu Y, Ochiai K, Kumon H, Huh NH, Watanabe M.
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Journal Title
Oncology Reports
Volume: 38
Issue: 2
Pages: 1108-1114
DOI
Related Report
Peer Reviewed / Open Access
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