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Analysis of biological functions of novel histone variants

Research Project

Project/Area Number 16K18479
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field System genome science
Research InstitutionKyushu University

Principal Investigator

Maehara Kazumitsu  九州大学, 生体防御医学研究所, 助教 (90726431)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsヒストンバリアント / エピゲノム制御 / ヒストンコード / クロマチン
Outline of Final Research Achievements

This project aimed to recognize the function of the novel histone variants in cell differentiation. In the mouse genome, we previously identified fourteen hitherto unknown H3 genes of which thirteen are analogous to H3.3, and reported that some of these genes were expressed in skeletal muscle. We demonstrated that one such histone H3 variant H3mm7 is preferentially expressed in quiescent satellite cells and that it is essential for myogenesis. Further chromatin analysis unveiled that H3mm7 was incorporated into the promoter regions of active gene loci including myogenic genes and that it was required for open chromatin structure to facilitate transcription. These results were published as well as three reports on human histone variants in our joint research projects.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (19 results)

All 2018 2017 2016 Other

All Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 12 results,  Open Access: 12 results,  Acknowledgement Compliant: 5 results) Presentation (3 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration2018

    • Author(s)
      Harada Akihito、Maehara Kazumitsu、Ono Yusuke、Taguchi Hiroyuki、Yoshioka Kiyoshi、Kitajima Yasuo、Xie Yan、Sato Yuko、Iwasaki Takeshi、Nogami Jumpei、Okada Seiji、Komatsu Tetsuro、Semba Yuichiro、Takemoto Tatsuya、Kimura Hiroshi、Kurumizaka Hitoshi、Ohkawa Yasuyuki
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 1400-1400

    • DOI

      10.1038/s41467-018-03845-1

    • NAID

      120006949474

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cryo-EM structure of the nucleosome containing the ALB1 enhancer DNA sequence2018

    • Author(s)
      Takizawa Yoshimasa、Tanaka Hiroki、Machida Shinichi、Koyama Masako、Maehara Kazumitsu、Ohkawa Yasuyuki、Wade Paul A.、Wolf Matthias、Kurumizaka Hitoshi
    • Journal Title

      Open Biology

      Volume: 8 Issue: 3 Pages: 170255-170255

    • DOI

      10.1098/rsob.170255

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sensitive detection of fluorescence in western blotting by merging images2018

    • Author(s)
      Kondo Yukari、Higa Shinichiro、Iwasaki Takeshi、Matsumoto Tomoya、Maehara Kazumitsu、Harada Akihito、Baba Yoshihiro、Fujita Masatoshi、Ohkawa Yasuyuki
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 1 Pages: 0191532-0191532

    • DOI

      10.1371/journal.pone.0191532

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The requirement of Mettl3-promoted MyoD mRNA maintenance in proliferative myoblasts for skeletal muscle differentiation2017

    • Author(s)
      Kudou Kensuke、Komatsu Tetsuro、Nogami Jumpei、Maehara Kazumitsu、Harada Akihito、Saeki Hiroshi、Oki Eiji、Maehara Yoshihiko、Ohkawa Yasuyuki
    • Journal Title

      Open Biology

      Volume: 7 Issue: 9 Pages: 170119-170119

    • DOI

      10.1098/rsob.170119

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chd2 regulates chromatin for proper gene expression toward differentiation in mouse embryonic stem cells2017

    • Author(s)
      Semba Yuichiro、Harada Akihito、Maehara Kazumitsu、Oki Shinya、Meno Chikara、Ueda Jun、Yamagata Kazuo、Suzuki Atsushi、Onimaru Mitsuho、Nogami Jumpei、Okada Seiji、Akashi Koichi、Ohkawa Yasuyuki
    • Journal Title

      Nucleic Acids Research

      Volume: 45 Issue: 15 Pages: 8758-8772

    • DOI

      10.1093/nar/gkx475

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal Structure and Characterization of Novel Human Histone H3 Variants, H3.6, H3.7, and H3.82017

    • Author(s)
      Taguchi H, Xie Y, Horikoshi N, Maehara K, Harada A, Nogami J, Sato K, Arimura Y, Osakabe A, Kujirai T, Iwasaki T, Semba Y, Tachibana T, Kimura H, Ohkawa Y, Kurumizaka H.
    • Journal Title

      Biochemistry

      Volume: 56 Issue: 16 Pages: 2184-2196

    • DOI

      10.1021/acs.biochem.6b01098

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Thymine DNA glycosylase modulates the DNA damage response and gene expression by base excision repair-dependent and -independent mechanisms2017

    • Author(s)
      Tomohumi Nakamura, Kouichi Murakami, Haruto Tada, Yoshihiko Uehara, Jumpei Nogami, Kazumitsu Maehara, Yasuyuki Ohkawa, Hisato Saitoh, Hideo Nishitani, Tetsuya Ono, Ryotaro Nishi, Masayuki Yokoi, Wataru Sakai, and Kaoru Sugasawa
    • Journal Title

      Genes to Cells

      Volume: 22 Issue: 4 Pages: 392-405

    • DOI

      10.1111/gtc.12481

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Crystal structure of the overlapping dinucleosome composed of hexasome and octasome2017

    • Author(s)
      Daiki Kato, Akihisa Osakabe, Yasuhiro Arimura, Yuka Mizukami, Naoki Horikoshi, Kazumi Saikusa, Satoko Akashi, Yoshifumi Nishimura, Sam-Yong Park, Jumpei Nogami, Kazumitsu Maehara, Yasuyuki Ohkawa, Atsushi Matsumoto, Hidetoshi Kono, Rintaro Inoue, Masaaki Sugiyama, Hitoshi Kurumizaka
    • Journal Title

      Science

      Volume: 356 Issue: 6334 Pages: 205-208

    • DOI

      10.1126/science.aak9867

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis2017

    • Author(s)
      Ueda J., Harada A., Urahama T., Machida S., Maehara K., Hada M., Makino Y., Nogami J., Horikoshi N., Osakabe A., Taguchi H., Tanaka H., Tachiwana H., Yao T., Yamada M., Iwamoto T., Isotani A., Ikawa M., Tachibana T., Okada Y., Kimura H., Ohkawa Y., Kurumizaka H., Yamagata K.
    • Journal Title

      Cell Reports

      Volume: 18(3) Issue: 3 Pages: 593-600

    • DOI

      10.1016/j.celrep.2016.12.065

    • NAID

      120006957416

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas.2016

    • Author(s)
      Kuniyoshi Y, Maehara K, Iwasaki T, Hayashi M, Semba Y, Fujita M, Sato Y, Kimura H, Harada A, Ohkawa Y.
    • Journal Title

      PLoS One

      Volume: 11 Issue: 10 Pages: e0165473-e0165473

    • DOI

      10.1371/journal.pone.0165473

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Histone H4 lysine 20 acetylation is associated with gene repression in human cells.2016

    • Author(s)
      Kaimori, J. Y., Maehara, K., Hayashi-Takanaka, Y., Harada, A., Fukuda, M., Yamamoto, S., Ichimaru, N., Umehara, T., Yokoyama, S., Matsuda, R., Ikura, T., Nagao, K., Obuse, C., Nozaki, N., Takahara, S., Takao, T., Ohkawa, Y., Kimura, H., and Isaka, Y.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 24318-24318

    • DOI

      10.1038/srep24318

    • NAID

      120005770760

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chromatin architecture may dictate the target site for DMC1, but not for RAD51, during homologous pairing2016

    • Author(s)
      Kobayashi W., Takaku M., Machida S., Tachiwana H., Maehara K., Ohkawa Y. and Kurumizaka H
    • Journal Title

      Scientific reports

      Volume: 6 Issue: 1 Pages: 24428-24428

    • DOI

      10.1038/srep24228

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ヒストンH3.3のサブバリアントH3mm7は正常な骨格筋再生に必要とされる2017

    • Author(s)
      前原 一満
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Myogenic Chromatin Structure Is Formed with the Histone H3 Variant H3mm72017

    • Author(s)
      Kazumitsu Maehara, Akihito harada, Jumpei Nogami, Yasuyuki Ohkawa
    • Organizer
      第20回生医研リトリート2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] ホッジ分解による擬似ライブ・シングルセルプロファイル追跡法2016

    • Author(s)
      前原一満
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Research-status Report
  • [Remarks] Ohkawa lab.

    • URL

      http://tx.bioreg.kyushu-u.ac.jp

    • Related Report
      2017 Annual Research Report
  • [Remarks] プレスリリース「筋肉の再生を促進させるスイッチの発見-筋肉の再生治療の応用に期待-」

    • URL

      https://www.kyushu-u.ac.jp/ja/researches/view/235

    • Related Report
      2017 Annual Research Report
  • [Remarks] 研究室HP

    • URL

      http://tx.bioreg.kyushu-u.ac.jp

    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 標的遺伝子の配列を決定する方法2017

    • Inventor(s)
      大川恭行、前原一満、木村宏、佐藤優子
    • Industrial Property Rights Holder
      九州大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017-03-16
    • Related Report
      2016 Research-status Report
    • Overseas

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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