Mechanistic insight into the ribosome quality control

• Project/Area Number: 16K18486
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: Tohoku University
• Principal Investigator: Matsuo Yoshitaka 東北大学, 薬学研究科, 助教 (00725252)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リボソーム / 品質管理 / 新生鎖 / mRNA / 新生ペプチド鎖

Outline of Final Research Achievements

Co-translational quality control system monitors mRNA translation and immediately eliminates aberrant mRNA and partially synthesized nascent peptides before translation is complete. Translation of poly(A) tail is one of the aborted translation in the cell, and generates poly-lysine peptide, inducing translation arrest and rapidly degradation of nascent peptides. Indeed, 12 polybasic amino acids coding segment such as poly-lysine and poly-arginine are efficient to induce ribosome stalling and subsequent proteasome mediated degradation of partially synthesized nascent peptide, which is called RQC: Ribosome Quality Control. E3 ubiquitin ligase Rqt1 ubiquitinates ribosomal protein S20 to induce RQC pathway, and we further have identified novel novel three factors (I named Rqt2, 3, 4, respectively) are enriched in the Rqt1-ribosome complex. Thus, we have analyzed the function of them in this project.

Research Products

• [Int'l Joint Research] University of Munich(ドイツ)
• [Int'l Joint Research] University of California, Berkeley(米国)
• [Journal Article] Ubiquitination of stalled ribosome triggers ribosome-associated quality control (2017)
  • Author(s): Matsuo Y, Ikeuchi K, Saeki Y, Iwasaki S, Schmidt C, Udagawa T, Sato F, Tsuchiya H, Becker T, Tanaka K, Ingolia NT, Beckmann R, Inada T
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 159-159
  • DOI: 10.1038/s41467-017-00188-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Ubiquitination of stalled ribosome triggers ribosome-associated quality control. (2017)
  • Author(s): Matsuo Y, Ikeuchi K, Saeki Y, Iwasaki S, Schmidt C, Udagawa T, Sato F, Tsuchiya H, Becker T, Tanaka K, Ingolia NT, Beckmann R, Inada T.
  • Organizer: 第19回日本RNA学会年会
• [Presentation] Mechanistic insight into recognition and dissociation of stalled ribosome to induce ribosome associated quality control system (2016)
  • Author(s): Yoshitaka Matsuo, Ken Ikeuchi, Yasushi Saeki, Christian Schmidt, Thomas Becker, Keiji Tanaka, Roland Beckmann and Toshifumi Inada
  • Organizer: 生化学会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2016-09-25
• [Presentation] Mechanistic insight into recognition and dissociation of stalled ribosome to induce ribosome associated quality control system (2016)
  • Author(s): Yoshitaka Matsuo, Ken Ikeuchi, Yasushi Saeki, Christian Schmidt, Thomas Becker, Keiji Tanaka, Roland Beckmann and Toshifumi Inada
  • Organizer: Ribosome meeting
  • Place of Presentation: 大阪医科大学 看護学部講堂
  • Year and Date: 2016-09-17
• [Presentation] Crucial roles of ribosome ubiquitination in quality controls and stress response (2016)
  • Author(s): Yoshitaka Matsuo, Toshifumi Inada
  • Organizer: 14th international congress on Yeast
  • Place of Presentation: Awaji Yumebutai
  • Year and Date: 2016-09-11
  • Int'l Joint Research