Function of novel strigolactone biosynthesis genes in Arabidopsis

• Project/Area Number: 16K18560
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Ehime University (2017-2018); Utsunomiya University (2016)
• Principal Investigator: Yoneyama Kaori 愛媛大学, 農学研究科, 助教 (20769997)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ストリゴラクトン / LBO / MAX1 / シロイヌナズナ / エンドウ / 地上部枝分れ抑制ホルモン

Outline of Final Research Achievements

Strigolactone (SL) is a shoot branching inhibitory plant hormone. The objective of this study is to elucidate the SL biosynthetic pathway and active shoot branching inhibitor by clarifying the function of novel SL biosynthetic genes. We reported that carlactone (CL) and methyl carlactonoate (MeCLA), SL intermediates accumulate in Arabidopsis lbo mutants and MeCLA is consumed as a substrate and [MeCLA+16 Da] compound is produced by recombinant LBO protein expressed in E. coli in PNAS paper (Brewer et al. 2016). Then, we found that [MeCLA+16 Da] is hydroxyMeCLA and exists in Arabidopsis SL insensitive mutants of Atd14 and not in lbo mutants (Yoneyama et al. in preparation). We also reported the function of MAX1 homologs, working upstream of LBO, in important major crops, model tree popular and model lower plant fern in New Phytologist (Yoneyama et al. 2018).

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により、SL生合成経路の全貌の解明に重要な知見を提供することができた。今後は、その地上部枝分かれ活性本体の解明により、代謝物の構造を元に、植物の地上部形態制御のための新たな農業資材の開発などにもつながるものと期待される。

Research Products

• [Int'l Joint Research] The University of Queensland(オーストラリア)
• [Journal Article] Conversion of carlactone to carlactonoic acid is a conserved function of MAX1 homologs in strigolactone biosynthesis. (2018)
  • Author(s): Yoneyama K, Mori N, Sato T, Yoda A, Xie X, Okamoto M, Iwanaga M, Ohnishi T, Nishiwaki H, Asami T, Yokota T, Akiyama K, Yoneyama K, Nomura T.
  • Journal Title: New Phytol. Volume: 218 Issue: 4 Pages: 1522-1533
  • DOI: 10.1111/nph.15055
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] LATERAL BRANCHING OXIDOREDUCTASE acts in the final stages of strigolactone biosynthesis in Arabidopsis (2016)
  • Author(s): Brewer, P.B., Yoneyama, K., Filardo, F., Meyers, E., Scaffidi, A., Frickey, T., Akiyama, K., Seto, Y., Dun, E.A., Cremer, J.E., Kerr, S.C., Waters, M.T., Flematti, G.R., Mason, M.G., Weiller, G., Yamaguchi, S., Nomura, T., Smith, S.M., Yoneyama, K., Beveridge, C.A.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 113 Issue: 22 Pages: 6301-6306
  • DOI: 10.1073/pnas.1601729113
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ストリゴラクトン生合成酵素LBOの機能解析 (2018)
  • Author(s): 米山香織
  • Organizer: 植物化学調節学会
• [Presentation] Hydroxycarlactone derivatives are potential substrates for MAX1 and LBO in strigolactone biosynthesis (2018)
  • Author(s): Kaori Yoneyama, Kohki Akiyama, Narumi Mori, Xiaonan Xie, Satoshi Yamauchi, Hisashi Nishiwaki, Koichi Yoneyama, Takahito Nomura
  • Organizer: 植物生理学会
• [Presentation] シロイヌナズナにおける内生ストリゴラクトンの同定 (2017)
  • Author(s): 米山香織、秋山康紀、森 愛美、謝 肖男、米山弘一、野村崇人
  • Organizer: 植物化学調節学会
• [Presentation] シロイヌナズナにおける新奇ストリゴラクトン生合成酵素LBOの機能解析 (2016)
  • Author(s): 米山香織
  • Organizer: 植物化学調節学会
  • Place of Presentation: 高知大学
  • Year and Date: 2016-10-28
• [Presentation] BIOCHEMICAL CHARACTERLIZATION OF LATERAL BRANCHING OXIDOREDUCTASE INVOLVING STRIGOLACTONE BIOSYNTHESIS IN ARABIDOPSIS (2016)
  • Author(s): Kaori Yoneyama
  • Organizer: Congress on International Plant Growth Substances
  • Place of Presentation: トロント、カナダ
  • Year and Date: 2016-06-21
  • Int'l Joint Research