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Development of Integrated Analysis Technique for Plant Phenome

Research Project

Project/Area Number 16K18562
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

Kutsuna Natsumaro  東京大学, 大学院新領域創成科学研究科, 特任准教授 (70578559)

Research Collaborator Hasezawa Seiichiro  
Higaki Takumi  
Akita Kae  
Kojo Kei H  
Shimahara Yuki  
Miura Takashi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords植物細胞 / オルガネラ / 細胞骨格 / 顕微鏡画像 / 画像解析 / 植物 / 細胞・組織 / 解析・評価 / 共焦点顕微鏡 / 生態生命情報学 / 生体生命情報学 / 発生・分化
Outline of Final Research Achievements

Plant cells have variety of organelle and cytoskeletal structures. To perform cellular level phenome analysis based on their microscopic images, integrated image processing software was developed in this research. Additionally, simulating software of cytoskeletal dynamics in plant cell was also developed. To collect imaging data, mainly tobacco culture cells BY-2 and transformant of BY-2 cells were observed, in addition to the microscopic images provided by other research institutions.

Academic Significance and Societal Importance of the Research Achievements

研究の結果、光学的特性等が自ずと異なる複数の顕微鏡撮影系に対して、オルガネラ等を認識し個数や面積を測定する、といった細胞レベルのフェノーム解析を高精度高効率に実施するソフトウェアを開発することができた。その基礎を形づくる画像解析ソフトウェア群については国内企業によるユーザインタフェイス等の改良等を受け、アップデートと提供を続けていけるような体制を整えている。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (33 results)

All 2019 2018 2017 2016 Other

All Journal Article (16 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 16 results,  Open Access: 11 results,  Acknowledgement Compliant: 4 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 6 results) Remarks (1 results)

  • [Journal Article] Quantitative Evaluation of Stromule Frequency at Hourly Intervals in <i>Arabidopsis</i> Stomatal Guard Cell Chloroplasts2019

    • Author(s)
      Shimahara Y, Kutsuna N, Hasezawa S, Kojo KH.
    • Journal Title

      CYTOLOGIA

      Volume: 84 Issue: 1 Pages: 31-35

    • DOI

      10.1508/cytologia.84.31

    • NAID

      130007628779

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2019-03-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A cloud-based bioimage analysis platform for morphological analysis and image classification.2019

    • Author(s)
      Shimahara Y, Sugawara K, Kojo KH, Kawai H, Yoshida Y, Hasezawa S, Kutsuna N
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 2 Pages: e0212619-e0212619

    • DOI

      10.1371/journal.pone.0212619

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ROSA26 reporter mouse lines and image analyses reveal the distinct region-specific cell behaviors in the visceral endoderm2018

    • Author(s)
      Abe T, Kutsuna N, Kiyonari H, Furuta Y, Fujimori T
    • Journal Title

      Development

      Volume: 145

    • DOI

      10.1242/dev.165852

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultrafast confocal fluorescence microscopy beyond the fluorescence lifetime limit2018

    • Author(s)
      Mikami H, Harmon J, Kobayashi H, Hamad S, Wang Y, Iwata O, Suzuki K, Ito T, Aisaka Y, Kutsuna N, Nagasawa K, Watarai H, Ozeki Y, Goda K.
    • Journal Title

      Optica

      Volume: 5 Issue: 2 Pages: 117-126

    • DOI

      10.1364/optica.5.000117

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation of uracil auxotroph mutants of coral symbiont alga for symbiosis studies2018

    • Author(s)
      Ishii Yuu、Maruyama Shinichiro、Fujimura-Kamada Konomi、Kutsuna Natsumaro、Takahashi Shunichi、Kawata Masakado、Minagawa Jun
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 3237-3237

    • DOI

      10.1038/s41598-018-21499-3

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Label-free detection of aggregated platelets in blood by machine-learning-aided optofluidic time-stretch microscopy.2017

    • Author(s)
      Jiang Y, Lei C, Yasumoto A, Kobayashi H, Aisaka Y, Ito T, Guo B, Nitta N, Kutsuna N, Ozeki Y, Nakagawa A, Yatomi Y, Goda K.
    • Journal Title

      Lab on a Chip

      Volume: 17 Issue: 14 Pages: 2426-2434

    • DOI

      10.1039/c7lc00396j

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Modification of growth anisotropy and cortical microtubule dynamics in Arabidopsis hypocotyls grown under microgravity conditions in space2017

    • Author(s)
      Soga K, Yamazaki C, Kamada M, Tanigawa N, Kasahara H, Yano S, Kojo KH, Kutsuna N, Kato T, Hashimoto T, Kotake T, Wakabayashi K, Hoson T
    • Journal Title

      Phyiologia Plantarum

      Volume: 162 Issue: 1 Pages: 135-144

    • DOI

      10.1111/ppl.12640

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Exogenous cellulase switches cell interdigitation to cell elongation in a RIC1-dependent manner in Arabidopsis thaliana cotyledon pavement cells.2017

    • Author(s)
      Higaki T, Takigawa-Imamura H, Akita K, Kutsuna N, Kobayashi R, Hasezawa S, Miura T
    • Journal Title

      Plant Cell Physiol

      Volume: 58 Pages: 106-119

    • DOI

      10.1093/pcp/pcw183

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] FIT: Statistical modeling tool for transcriptome dynamics under fluctuating field conditions2017

    • Author(s)
      Iwayama K., Aisaka Y., Kutsuna N., Nagano A.J.
    • Journal Title

      Bioinformatics

      Volume: - Issue: 11 Pages: 1672-1680

    • DOI

      10.1093/bioinformatics/btx049

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] ブドウ挿し木苗および幼苗へのブドウ黒とう病菌噴霧接種による抵抗性評価法の開発2017

    • Author(s)
      河野淳, 朽名夏麿, 尾上典之, 伴雄介, 佐藤明彦
    • Journal Title

      園芸学研究

      Volume: 印刷中

    • NAID

      130006300701

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dopamine regulates body size in Caenorhabditis elegans2016

    • Author(s)
      Takashi Nagashima, Eitaro Oamia, Natsumaro Kutsuna, Shoichi Ishiura, Satoshi Suo
    • Journal Title

      Developmenta Biology

      Volume: 412 Issue: 1 Pages: 128-138

    • DOI

      10.1016/j.ydbio.2016.02.021

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A Theoretical Model of Jigsaw-Puzzle Pattern Formation by Plant Leaf Epidermal Cells2016

    • Author(s)
      Takumi Higaki, Natsumaro Kutsuna, Kae Akita, Hisako Takigawa-Imamura, Kenji Yoshimura, Takashi Miura
    • Journal Title

      PLOS Computational Biology

      Volume: 12 Issue: 4 Pages: e1004833-e1004833

    • DOI

      10.1371/journal.pcbi.1004833

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of image manipulation and plagiarism in life sciences research.2016

    • Author(s)
      Shimahara Y, Kutsuna N
    • Journal Title

      Editorial Office News

      Volume: 9 Issue: 4 Pages: 1-2

    • DOI

      10.18243/eon/2016.9.4.5

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Modulation of plant RAB GTPase-mediated membrane trafficking pathway at the interface between plants and obligate biotrophic pathogens.2016

    • Author(s)
      Inada, N., Betsuyaku, S., Shimada, T., Ebine, K., Ito, E., Kutsuna, N., Hasezawa, S., Takano, Y., Fukuda, H., Nakano, A., & Ueda, T.
    • Journal Title

      Plant Cell Physiol.

      Volume: 57 Issue: 9 Pages: 1854-1864

    • DOI

      10.1093/pcp/pcw107

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] In vivo 3D analysis of systemic effects after local heavy-ion beam irradiation in an animal model2016

    • Author(s)
      Nagata K, Hashimoto C, Watanabe-Asaka T, Itoh K, Yasuda T, Ohta K, Oonishi H, Igarashi K, Suzuki M, Funayama T, Kobayashi Y, Nishimaki T, Katsumura T, Oota H, Ogawa M, Oga A, Ikemoto K, Itoh H, Kutsuna N, Oda S, and Mitani H
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 28691-28691

    • DOI

      10.1038/srep28691

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated apoplastic space in Arabidopsis leaf epidermis2016

    • Author(s)
      Akita, K., Kobayashi, M., Sato, M., Kutsuna, N., Ueda, T., Toyooka, K., Nagata, N., Hasezawa, S., and Higaki, T.
    • Journal Title

      Protoplasma

      Volume: in press Issue: 1 Pages: 367-377

    • DOI

      10.1007/s00709-016-0955-1

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Enteroendocrine peptides can regulate insect feeding behavior via controlling intestinal contraction.2018

    • Author(s)
      Matsumoto Sumihiro, Kutsuna Natsumaro, Nagata Shinji
    • Organizer
      Asia and Oceania Society for Comparative Endocrinology Intercongress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] AIによる画像解析: 植物科学への応用2018

    • Author(s)
      朽名夏麿, 島原佑基, 馳澤盛一郎
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Feedback regulation in secondary cell wall thickening in poplar2018

    • Author(s)
      Naoki Takata, Tatsuya Awano, Natsumaro Kutsuna, Toru Taniguchi
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 子葉表皮細胞壁の湾曲における微小管結合タンパク質RIC1の役割: 細胞形態計測と力学モデルによる解析2017

    • Author(s)
      桧垣匠, 今村寿子, 秋田佳恵, 朽名夏麿, 三浦岳, 馳澤 盛一郎
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 非標識リアルタイムイメージング装置を用いた血小板凝集塊の検出法の確立2017

    • Author(s)
      安本篤史, Yiyue Jiang, Cheng Lei, 相坂有理, 伊藤卓朗, Baoshan Guo, 小林博文, 新田尚, 朽名夏麿, 小関泰之, 中川敦寛, 合田圭介, 矢冨裕
    • Organizer
      第39回日本血栓止血学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] バイオメディカル画像の分類・評価のための人工知能によるアプローチ2017

    • Author(s)
      朽名夏麿
    • Organizer
      先進歯科画像研究会第一回学術講演
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 生物画像自動分類を用いたシロイヌナズナ色素体におけるストロミュールの検出2017

    • Author(s)
      島原佑基, 湖城恵, 馳澤盛一郎, 朽名夏麿
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 人工知能による植物の効率的フェノタイピング2017

    • Author(s)
      朽名夏麿
    • Organizer
      第35回日本植物細胞分子生物学会大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 宇宙の微小重力環境におけるシロイヌナズナ胚軸の成長と表層微小管動態 - Aniso Tubule 宇宙実験2017

    • Author(s)
      曽我康一, 山崎千秋, 鎌田源司, 谷川直樹, 笠原春夫, 矢野幸子, 湖城恵, 朽名夏麿, 加藤壮英, 橋本隆, 小竹敬久, 若林和幸, 保尊隆享
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 生物画像の自動評価工程をハイスループット化する能動学習技術 CARTA2017

    • Author(s)
      朽名夏麿
    • Organizer
      定量生物学の会 第8回年会
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Mechanism of jigsaw-puzzle pattern formation in plant leaf epidermal cell: modeling single cell shape by interface equation and convolution kernel.2016

    • Author(s)
      Miura T, Higaki T, Akita K, Kutsuna N, Takigawa-Imamura H.
    • Organizer
      The 2016 (26th) annual meeting of the Japanese Society for Mathematical Biology
    • Place of Presentation
      九州大学伊都新キャンパス(福岡県福岡市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 生物・医用画像の自動評価のための能動的機械学習法 CARTA2016

    • Author(s)
      朽名夏麿
    • Organizer
      第20回 NMR マイクロイメージング研究会
    • Place of Presentation
      国立がん研究センター東病院(千葉県柏市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Radiomics 入門: 機械学習の基礎2016

    • Author(s)
      朽名夏麿
    • Organizer
      第44回 日本磁気共鳴医学会大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県さいたま市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] マウス着床後初期胚における細胞系譜解析2016

    • Author(s)
      Takaya Abe, Natsumaro Kutsuna, Hiroshi Kiyonari, Yasuhide Furuta, and Toshihiko Fujimori
    • Organizer
      日本発生生物学会大会 秋季シンポジウム2016
    • Place of Presentation
      三島市民文化会館(静岡県三島市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 着床後マウス胚における細胞系譜解析2016

    • Author(s)
      阿部高也、朽名夏麿、清成寛、古田 泰秀、藤森 俊彦
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 植物表皮細胞の湾曲構造形成のメカニズム2016

    • Author(s)
      三浦岳、桧垣匠、朽名夏麿、秋田佳恵、今村(滝川) 寿子、吉村 賢二
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2016 Research-status Report
  • [Remarks] 東京大学大学院新領域創成科学研究科植物全能性制御システム解析学分野ウェブページ

    • URL

      http://hasezawa.ib.k.u-tokyo.ac.jp/zp/hlab/

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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