Development of a mathematical method for analyzing deformation dynamics of living tissues by 4D variational analysis

• Project/Area Number: 16K18578
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Ehime University
• Principal Investigator: Saitou Takashi 愛媛大学, 医学部附属病院, 助教(病院教員) (60588705)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 形態形成 / シミュレーション / バイオイメージング / 小型魚類 / 胚発生 / ライブイメージング / データ同化

Outline of Final Research Achievements

Development of advanced techniques that fully utilize information from experimental data is a challenging task. In this study, we aimed to advance integration of four dimensional measurement data obtained through fluorescent live imaging analysis and numerical simulation data from mathematical models. Applying the method to the time series data describing cell state changes, deformation dynamics of living tissues were investigated.

Research Products

• [Journal Article] In vivo optical imaging of cancer cell function and tumor microenviroment (2018)
  • Author(s): Takeshi Imamura, Takashi Saitou, Ryosuke Kawakami
  • Journal Title: Cancer Science Volume: 109 Issue: 4 Pages: 912-918
  • DOI: 10.1111/cas.13544
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative Morphometry for Osteochondral Tissues Using Second Harmonic Generation Microscopy and Image Texture Information. (2018)
  • Author(s): Saitou T, Kiyomatsu H, Imamura T.
  • Journal Title: Sci Rep. Volume: 8(1) Issue: 1 Pages: 2826-2826
  • DOI: 10.1038/s41598-018-21005-9
  • Peer Reviewed / Open Access
• [Journal Article] 医療応用を目指した愛媛大学医学部発の蛍光イメージング技術開発 (2018)
  • Author(s): 今村健志，審良太郎，明比麻由，下山佳織，古賀繁宏，渡部祐司，清松悠，三浦裕正，山本晋，日浅陽一，齋藤卓
  • Journal Title: 愛媛医学 Volume: 36 Pages: 123-127
  • Peer Reviewed
• [Journal Article] The HIV co-receptor CCR5 regulates osteoclast function. (2017)
  • Author(s): Lee JW, Hoshino A, Inoue K, Saitou T, Uehara S, Kobayashi Y, Ueha S, Matsushima K, Yamaguchi A, Imai Y, Iimura T.
  • Journal Title: Nat Commun Volume: 8 Issue: 1 Pages: 2226-2226
  • DOI: 10.1038/s41467-017-02368-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Quantitative imaging of fibrotic and morphological changes in liver of non-alcoholic steatohepatitis (NASH) model mice by second harmonic generation (SHG) and auto-fluorescence (AF) imaging using two-photon excitation microscopy (TPEM). (2016)
  • Author(s): Yamamoto S, Oshima Y, Saitou T, Watanabe T, Miyake T, Yoshida O, Tokumoto Y, Abe M, Matsuura B, Hiasa Y, Imamura T
  • Journal Title: Biochemistry and Biophysics Reports Volume: 8 Pages: 277-283
  • DOI: 10.1016/j.bbrep.2016.09.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 第２高調波発生イメージングを用いた骨・軟骨組織の計量病理学 (2017)
  • Author(s): 齋藤卓
  • Organizer: 日本応用数理学会２０１７年度年会
  • Invited
• [Presentation] Mathematical inference of spatiotemporal cell cycle patterns in zebrafish body axis development (2017)
  • Author(s): Takashi Saitou
  • Organizer: The 23rd Japanese Medaka and Zebrafish Meeting
  • Int'l Joint Research
• [Presentation] 変形性関節症モデルマウスと非線形光イメージングによる関節軟骨変性の定量化法の開発 (2016)
  • Author(s): 齋藤卓
  • Organizer: 平成２８年度 先端モデル動物支援 若手支援技術講習会
  • Place of Presentation: 蓼科グランドホテル滝の湯
  • Year and Date: 2016-09-14
• [Presentation] 細胞周期ライブイメージングとゼブラフィッシュ形態形成の定量解析 (2016)
  • Author(s): 齋藤卓
  • Organizer: 第６８回日本細胞生物学会大会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2016-06-15
  • Invited
• [Book] 実験医学増刊 Vol.35 No.5, 187-191 (2017)
  • Author(s): 齋藤卓，今村健志
  • Total Pages: 5
  • Publisher: 羊土社
• [Book] 実験医学特集 Vol.34 No.14, 2344-2347 (2016)
  • Author(s): 今村健志，齋藤卓
  • Total Pages: 4
  • Publisher: 羊土社