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Functional studies of KNOX-BLH complex in rice meristems.

Research Project

Project/Area Number 16K18637
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Science in genetics and breeding
Research InstitutionNational Institute of Genetics

Principal Investigator

Tsuda Katsutoshi  国立遺伝学研究所, 遺伝形質研究系, 助教 (30756408)

Research Collaborator HAKE Sarah  カリフォルニア大学, バークレー校
MAENO Akiteru  国立遺伝学研究所
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywordsメリステム / 介在分裂組織 / 節間伸長 / 転写因子 / 茎頂分裂組織 / KNOX転写因子 / BLH転写因子 / イネ / 発現制御 / 成長・発生遺伝 / 遺伝子ネットワーク
Outline of Final Research Achievements

We investigated the function of BLH transcription factors in rice and maize. Maize blh12/14 mutants showed unique phenotypes such as shortened internode and precocious anastomosis in the stem, suggesting BLH TFs play important roles in stem development. Rice blh mutants showed extremely shortened internode phenotypes. We carried out transcriptome analyses and identified several genes/pathways that are specifically working in the intercalary meristems. These BLH downstream genes will be keys to understand internode development, which is important to regulate plant height.
During these studies, we developed a method to observe internal structure using micro CT. This method can be used to obtain 3D modeling of various plant organs, as exemplified by the study of BLH function in the rice panicle development.

Academic Significance and Societal Importance of the Research Achievements

植物は光獲得のために茎を伸ばす一方、過剰な身長は植物体の倒伏を招くため、適切な茎伸長制御は農業上重要な課題であるが、節間伸長機構はほとんど未解明であった。本研究では、重要穀物であるイネ・トウモロコシにおけるBLH転写因子の機能解析を行なった結果、これらの因子が節間を生み出す介在分裂組織形成・維持に不可欠な役割を果たすことがわかった。また、マイクロCTを用いた観察法を確立し、従来は困難であった茎の内部構造の全体像を把握することが可能となった。
現在BLHの下流遺伝子経路が絞り込めて来ており、本研究の成果は将来的な穀物の節間成長制御のための重要な基盤となることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Int'l Joint Research] University of California, Berkeley(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] BELL1-like homeobox genes regulate inflorescence architecture and meristem maintenance in rice2019

    • Author(s)
      Ikeda Takuyuki、Tanaka Wakana、Toriba Taiyo、Suzuki Chie、Maeno Akiteru、Tsuda Katsutoshi、Shiroishi Toshihiko、Kurata Tetsuya、Sakamoto Tomoaki、Murai Masayuki、Matsusaka Hiroaki、Kumamaru Toshihiro、Hirano Hiro-Yuki
    • Journal Title

      The Plant Journal

      Volume: 印刷中 Issue: 3 Pages: 465-478

    • DOI

      10.1111/tpj.14230

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Micro-computed Tomography to Visualize Vascular Networks in Maize Stems2018

    • Author(s)
      Maeno Akiteru、Tsuda Katsutoshi
    • Journal Title

      BIO-PROTOCOL

      Volume: 7 Issue: 1

    • DOI

      10.21769/bioprotoc.2682

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] KNOTTED1 cofactors, BLH12 and BLH14, regulate internode patterning and vein anastomosis in maize.2017

    • Author(s)
      Katsutoshi Tsuda, Maria-Jazmin Abraham-Juarez, Akiteru Maeno, Zhaobin Dong, Dale Aromdee, Robert Meeley, Toshihiko Shiroishi, Ken-ichi Nonomura and Sarah Hake
    • Journal Title

      The Plant Cell

      Volume: 印刷中 Issue: 5 Pages: 1105-1118

    • DOI

      10.1105/tpc.16.00967

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Control of subcellular localization of a KNOX transcription factor in rice.2019

    • Author(s)
      津田勝利、野々村賢一
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Visualization of vascular networks in maize stems using micro-CT2018

    • Author(s)
      Akiteru Maeno and Katsutoshi Tsuda
    • Organizer
      日本育種学会 第133回講演会(九州大学)
    • Related Report
      2017 Research-status Report
  • [Presentation] A role of KNOX-BLH transcription factors in scattered vein patterning in grasses2017

    • Author(s)
      Katsutoshi Tsuda, Maria-Jazmin Abraham-Juarez, Akiteru Maeno, Zhaobin Dong, Dale Aromdee, Robert Meeley, Toshihiko Shiroishi, Ken-ichi Nonomura and Sarah Hake
    • Organizer
      日本植物生理学会
    • Place of Presentation
      鹿児島大学(鹿児島)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Regulation of scattered venation and node/internode patterning in maize.2016

    • Author(s)
      Katsutoshi Tsuda
    • Organizer
      日本発生生物学会秋季シンポジウム
    • Place of Presentation
      三島市民文化会館(三島)
    • Year and Date
      2016-10-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Regulation of scattered venation and internode patterning in maize2016

    • Author(s)
      津田勝利, ドン ジャオビン, アブラハム マリア, 前野哲輝, アロムディ デイル, ミーリー ロバート, 城石俊彦, 野々村賢一, ヘイク サラ
    • Organizer
      日本植物学会
    • Place of Presentation
      沖縄コンベンションセンター(宜野湾)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Chromatin dynamics of the knotted1 locus during shoot development2016

    • Author(s)
      Katsutoshi Tsuda and Sarah Hake
    • Organizer
      Plant Genome Stability and Change 2016
    • Place of Presentation
      湘南国際村(葉山)
    • Year and Date
      2016-07-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 単子葉植物の茎に特徴的な形態形成を制御するメカニズム

    • URL

      https://www.nig.ac.jp/nig/ja/2017/04/research-highlights_ja/20170410-2.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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