Development of markers for resistance to anthracnose in new strawberry cultivar by RAD-Seq

Research Project

Project/Area Number	16K18638
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Science in genetics and breeding
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	HIKAWA-ENDO Minori 国立研究開発法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター, 研究員 (90614652)
Research Collaborator	KIM Sangwan TASHIRO Kosuke KUHARA Satoru MORIWAKI Jouji
Project Period (FY)	2016-04-01 – 2019-03-31
Project Status	Completed (Fiscal Year 2018)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	イチゴ / イチゴ炭疽病 / 新品種 / 病害抵抗性 / RAD-seq解析 / DNAマーカー / 炭疽病 / 次世代シーケンサー / RAD-Seq / 抵抗性 / C. gloeosporioides / C. siamense / C. fructicola / Colletotrichum属 / 育種学 / 遺伝子
Outline of Final Research Achievements	Strawberry anthracnose resistance of Japanese new strawberry cultivar 'Koiminori' as was investigated; It was found that 'Koiminori' was resistant to Colletotrichum siamense but susceptible to C. fructicola. Our studies are the first to demonstrate separate anthracnose resistances of strawberry plants. RNA-Seq experiments using two groups of F1 seedlings were also conducted to identify two resistance genes.
Academic Significance and Societal Importance of the Research Achievements	イチゴ炭疽病はいくつかの病原菌により引き起こされるイチゴ生産における最重要病害であり、激発時には生産者に甚大な経済的損失を与える。本研究では、従来知られるイチゴ炭疽病抵抗性は一部の病原菌には効果を有さないことを示し、複数の抵抗性を兼ね備えることの重要性を初めて示した。また、新品種「恋みのり」にこれまで知られなかった抵抗性を見出し、イチゴの病害抵抗性育種の進歩に貢献した。