The rational design and development of anti-venom drugs for snakebites based on the endogenous inhibitors from Japanese Viper
Project/Area Number |
16K18880
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biological pharmacy
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Research Institution | Fukuoka University |
Principal Investigator |
Shioi Narumi 福岡大学, 理学部, 助教 (50510187)
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Research Collaborator |
Maenaka Katsumi
Kurahara Lin Hai
Hu Yaopeng
Hiraishi Keizo
Hirano Toru
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ヘビ毒タンパク質 / ヘビ血清タンパク質 / 毒素阻害剤 / イオンチャネルブロッカー / ヘビ毒素タンパク質 / ハブ血清タンパク質 / 生体防御機構 / タンパク質性毒素 / 毒ヘビ血液タンパク質 / 金属プロテアーゼ / プロテアーゼ阻害剤 |
Outline of Final Research Achievements |
According to WHO report is estimated 5.4 million people are bitten each year. Snakebite is a serious problem as a neglected public health issue in the world. A crude venom contains various proteins induced various effects in their prey or their human victim. On the other hand, venomous snakes have endogenous proteins to neutralize the toxicity of their venom components. We identified new class of endogenous inhibitors from Protobothrops flavoviridis serum. In this study, we investigated of potential utility of SSPs in therapeutic drug for snakebites as follows; (1) we have identified target ion channels of ion channel blockers of Japanese Viper and revealed that venom snake serum protein inhibits the toxin's physiological activity. (2) Peptides synthesis of the toxin binding domains of the inhibitory proteins were carried out using a phage display method and chemical synthesis method. (3) We identified binding proteins from several snake crude venoms using by snake blood components.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Haemorrhagic snake venom metalloproteases and human ADAMs cleave LRP5/6, which disrupts cell-cell adhesions in vitro and induces haemorrhage in vivo.2017
Author(s)
Seo T, Sakon T, Nakazawa S, Nishioka A, Watanabe K, Matsumoto K, Akasaka M, Shioi N, Sawada H, Araki S
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Journal Title
FEBS Journal
Volume: 284
Issue: 11
Pages: 1657-1671
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 毒ヘビの血液成分について2016
Author(s)
加藤誠、佐藤晴菜、岩崎翔、塩井成留実
Organizer
第63回トキシンシンポジウム
Place of Presentation
滝の湯(山形県天童市)
Year and Date
2016-07-14
Related Report
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