| Project/Area Number |
16K18928
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Rimbara Emiko 国立感染症研究所, 細菌第二部, 主任研究官 (20349822)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | Helicobacter cinaedi / Autotransporter protein / autotransporter protein / Adhesion / RGD / 病原因子 / 細菌 |
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| Outline of Final Research Achievements |
Helicobacter cinaedi infects colon in human and animals, and causes bacteremia and cellulitis in human. To identify a virulence factor of H. cinaedi, we focused on an autotransporter protein X of H. cinaedi in this study.
Structure of protein X is predicted to be similar to Serine Protease Autotransporters of Enterobacteriaceae（SPATEs), most of which are secreted. However, protein X is suggested to remain on bacterial surface by immunoblotting assay using peptide antibody against protein X.
By in vitro infection experiments using protein X knockout strains, protein X was found to be contribute to cell adherance. In addition we found the RGD motif in protein X may play a important role to adehere cell.
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| Academic Significance and Societal Importance of the Research Achievements |
Helicobacter cinaediは近年その分離が増加している病原細菌である．さらにH. cinaediは人から人へと伝播し院内感染を引き起こす．病原因子としてcytolethal distending toxin（CDT）を持つことがわかっているが，それ以外の病原因子はほとんど明らかになっていなかった．本研究ではAutotransporter proteinが細胞接着に寄与することによりH. cinaediの感染病態に関連する病原因子であることが示唆された．
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