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Synapse maintenance by afadin and its binding proteins

Research Project

Project/Area Number 16K19036
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionKobe University

Principal Investigator

Maruo Tomohiko  神戸大学, 医学研究科, 医学研究員 (10625114)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords海馬苔状線維シナプス / 加齢 / アファディン / ネクチン / マギン / シナプス / パンクタアドヘレンティアジャンクション / 神経シナプス
Outline of Final Research Achievements

The investigations using knockout mice of afadin and its binding protein MAGUIN revealed that proteins localized at puncta adherentia junctions (PAJs) regulate synapse formation, maintenance, and resultant functions. On the other hand, synaptic junctions and PAJs are unexpectedly stable in the aged mouse hippocampus under the normal housing condition. There were quantitative alterations, to some extent, of PAJ protein accumulation at the sites of the hippocampal giant mossy fiber synapses, although further experiments would be required to clarify the functional significance of these changes. Toward this aim, I will study aged-afadin and MAGUIN knockout mice, and mouse models in which PAJ protein accumulation is affected.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (13 results)

All 2018 2017 2016

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Acknowledgement Compliant: 1 results) Presentation (6 results)

  • [Journal Article] Requirement of the F-actin-binding activity of l-afadin for enhancing the formation of adherens and tight junctions2018

    • Author(s)
      Sakakibara Shotaro、Maruo Tomohiko、Miyata Muneaki、Mizutani Kiyohito、Takai Yoshimi
    • Journal Title

      Genes to Cells

      Volume: 23 Issue: 3 Pages: 185-199

    • DOI

      10.1111/gtc.12566

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple Roles of Afadin in the Ultrastructural Morphogenesis of Mouse Hippocampal Mossy Fiber Synapses.2017

    • Author(s)
      Sai K, Wang S, Kaito A, Fujiwara T, Maruo T, Itoh Y, Miyata M, Sakakibara S, Miyazaki N, Murata K, Yamaguchi Y, Haruta T, Nishioka H, Motojima Y, Komura M, Kimura K, Mandai K, Takai Y, Mizoguchi A.
    • Journal Title

      J Comp Neurol

      Volume: - Issue: 12 Pages: 2719-2734

    • DOI

      10.1002/cne.24238

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of afadin in functional differentiations of hippocampal mossy fiber synapse2017

    • Author(s)
      Geng Xiaoqi、Maruo Tomohiko、Mandai Kenji、Supriyanto Irwan、Miyata Muneaki、Sakakibara Shotaro、Mizoguchi Akira、Takai Yoshimi、Mori Masahiro
    • Journal Title

      Genes to Cells

      Volume: 22 Issue: 8 Pages: 715-722

    • DOI

      10.1111/gtc.12508

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NGL-3-induced presynaptic differentiation of hippocampal neurons in an afadin-dependent, nectin-1-independent manner2017

    • Author(s)
      Maruo Tomohiko、Mandai Kenji、Miyata Muneaki、Sakakibara Shotaro、Wang Shujie、Sai Kousyoku、Itoh Yu、Kaito Aika、Fujiwara Takeshi、Mizoguchi Akira、Takai Yoshimi
    • Journal Title

      Genes to Cells

      Volume: 22 Issue: 8 Pages: 742-755

    • DOI

      10.1111/gtc.12510

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of afadin in the formation of the cellular architecture of the mouse hippocampus and dentate gyrus.2017

    • Author(s)
      Miyata M, Maruo T, Kaito A, Wang S, Yamamoto H, Fujiwara T, Mizoguchi A, Mandai K, Takai Y
    • Journal Title

      Mol Cell Neurosci

      Volume: 79 Pages: 34-44

    • DOI

      10.1016/j.mcn.2016.12.007

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aging-dependent expression of synapse-related proteins in the mouse brain.2017

    • Author(s)
      Shiotani, H., Maruo, T., Sakakibara, S., Miyata, M., Mandai, K., Mochizuki, H., Takai, Y.
    • Journal Title

      Genes to Cells

      Volume: 印刷中 Issue: 5 Pages: 472-484

    • DOI

      10.1111/gtc.12489

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Localization of nectin-2δ at perivascular astrocytic endfoot processes and degeneration of astrocytes and neurons in nectin-2 knockout mouse brain.2016

    • Author(s)
      Miyata M, Mandai K, Maruo T, Sato J, Shiotani H, Kaito A, Itoh Y, Wang S, Fujiwara T, Mizoguchi A, Takai Y, Rikitake Y
    • Journal Title

      Brain Res

      Volume: 1649 Pages: 90-101

    • DOI

      10.1016/j.brainres.2016.08.023

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 海馬神経細胞のシナプス前部の分子的および機能的な分化におけるアファディンの関与2017

    • Author(s)
      丸尾 知彦、萬代 研二、宮田 宗明、榊原 正太郎、王 淑杰、崔 煌植、伊藤 優、垣内 愛加、藤原 武志、耿 瀟奇、イルワン スプリヤント、溝口 明、森 正弘、高井 義美
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脳におけるネクチン-2の局在と機能2017

    • Author(s)
      宮田 宗明、萬代 研二、丸尾 知彦、佐藤 淳哉、塩谷 元、垣内 愛加、伊藤 優、王 淑杰、藤原 武志、溝口 明、高井 義美、力武 良行
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マウス網膜におけるNectin-1を介したSelf-to-Self新規接着機構の解析2017

    • Author(s)
      王 淑杰、垣内 愛加 、崔 煌植、萬代 研二、丸尾 知彦、高井 義美、溝口 明
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 海馬苔状線維シナプス分化における2つのアファディンスプライスバリアントの役割.2016

    • Author(s)
      丸尾 知彦, 萬代 研二, 榊原 正太郎, 伊藤 優, 藤原 武志, 王 淑杰, 崔 煌植, 垣内 愛加, 耿 瀟奇, 森 正弘, 溝口 明, 高井 義美.
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] 海馬発生におけるアファディンの役割.2016

    • Author(s)
      宮田 宗明, 丸尾 知彦, 山本 昆明, 垣内 愛加, 王 淑杰, 藤原 武志, 溝口 明, 萬代 研二, 高井 義美.
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report
  • [Presentation] ネクチン-2αとマウス手綱核.2016

    • Author(s)
      塩谷 元, 萬代 研二, 宮田 宗明, 丸尾 知彦, 垣内 愛加, 王 淑杰, 藤原 武志, 溝口 明, 望月 秀樹, 高井 義美.
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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