Study on the involvement of collectin CL-K1 in the pathogenesis of 3MC syndrome and elucidation of its molecular mechanism

• Project/Area Number: 16K19045
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Asahikawa Medical College
• Principal Investigator: Matsuda Yasuyuki 旭川医科大学, 医学部, 助教 (10532252)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: コレクチン / 3MC症候群 / 発生 / 自然免疫

Outline of Final Research Achievements

The association between the gene defect of collectin CL-K1 and the pathology of 3MC syndrome has been found, suggesting that CL-K1 may be involved in growth and development. The purpose of this study is to elucidate the pathogenesis of 3MC syndrome in order to explore the possibility of establishing a medical treatment for it. I found that CL-K1 interacts with collectin CL-L1, suggesting that CL-L1 may also be related to the pathogenesis of 3MC syndrome.

Academic Significance and Societal Importance of the Research Achievements

3MC症候群の発症原因は不明なままであり、根本的な治療法や予防法の確立には至っていない。本研究の目的は、3MC症候群で生じる病態やその発症機序を明らかにすることで、治療法や予防法開発の糸口となる知見を得るところにある。本研究成果では、3MC症候群の発症に別のコレクチンCL-L1も関与する可能性があることを示唆することができた。この成果は、本疾患の将来的な治療法、予防法開発において有用な情報となる可能性が考えられる。

Research Products

• [Journal Article] Plasminogen Tochigi mice exhibit phenotypes similar to wild-type mice under experimental thrombotic conditions (2017)
  • Author(s): Tashima Yuko、Banno Fumiaki、Kita Toshiyuki、Matsuda Yasuyuki、Yanamoto Hiroji、Miyata Toshiyuki
  • Journal Title: PLOS ONE Volume: 12 Issue: 7 Pages: e0180981-e0180981
  • DOI: 10.1371/journal.pone.0180981
  • Peer Reviewed / Open Access
• [Journal Article] Three pentraxins C-reactive protein, serum amyloid p component and pentraxin 3 mediate complement activation using Collectin CL-P1 (2017)
  • Author(s): 2.Roy, N., Ohtani, K., Hidaka, Y., Amano, Y., Matsuda, Y., Mori, K., Hwang, I., Inoue, N., Wakamiya, N.
  • Journal Title: Biochim Biophys Acta Volume: 861 Issue: 2 Pages: 1-14
  • DOI: 10.1016/j.bbagen.2016.11.023
  • NAID: 120006715888
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Collectin kidney 1 plays an important role in innate immunity against Streptococcus pneumoniae infection. (2017)
  • Author(s): Hwang I, Mori K, Ohtani K, Matsuda Y, Roy N, Kim Y, Suzuki Y, Wakamiya N
  • Journal Title: J Innate Immun Volume: 9 Issue: 2 Pages: 217-228
  • DOI: 10.1159/000453316
  • NAID: 120006711670
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Collectin CL-P1 utilizes C-reactive protein for complement activation. (2016)
  • Author(s): 5.Roy, N., Ohtani, K., Matsuda, Y., Mori, K., Hwang, I., Suzuki, Y., Inoue, N., Wakamiya, N.
  • Journal Title: Biochim Biophys Acta Volume: 1860 Issue: 6 Pages: 1118-1128
  • DOI: 10.1016/j.bbagen.2016.02.012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Collectin CL-LK activates the complement system via binding to DNA (2017)
  • Author(s): Yasuyuki Matsuda, Katsuki Ohtani, Nitai Roy, Kenichiro Mori, Insu Hwang, Nobutaka Wakamiya
  • Organizer: 2017年度生命科学系学会合同年次大会 (第40回日本分子生物学会年会、第90回日本生化学会大会)
• [Presentation] Structural characterization of novel collectins, CL-L1, CL-K1, and CL-LK in blood (2016)
  • Author(s): Yasuyuki Matsuda, Katsuki Ohtani, Nitai Roy, Insu Hwang, Kenichiro Mori, Nobutaka Wakamiya
  • Organizer: XXVIth INTERNATIONAL COMPLEMENT WORKSHOP
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2016-09-04
  • Int'l Joint Research