| Project/Area Number |
16K19123
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ボツリヌス / 神経毒素複合体 / 腸管吸収 / M細胞 / 免疫細胞 / HA / 神経毒素 / 樹状細胞 / 細菌毒素 |
|---|
| Outline of Final Research Achievements |
Food-borne botulism is caused by ingestion of botulinum neurotoxin (BoNT) complexes which composed of BoNT and one or more neurotoxin-associated proteins (NAPs). We have uncovered the site(s) and mechanism of intestinal absorption of type A1 BoNT complex, heretofore largely unknown, which is essential for the onset of food-borne botulism.
In this study, we found that type B1 BoNT complex invades into the host from intestine by different mechanisms from type A1. Furthermore, we observed that both type A and type B BoNT complex interact with immune cells which are located in sub-epithelial dome (SED). These results suggest that intestinal immune cells influence the entry of type A1 or type B1 BoNT into the blood stream and are involved in the onset of food-borne botulism.
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