| Project/Area Number |
16K19128
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
Hinenoya Atsushi 大阪府立大学, 生命環境科学研究科, 准教授 (20548490)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Escherichia albertii / shiga toxin / Stx2f / mice / animal model / マウス / 腎臓傷害 / Shiga toxin 2f / 病原性 / stx2f / in vivo |
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| Outline of Final Research Achievements |
We tried to evaluate the virulence property of Stx2f-producing Escherichia albertii. Successfully, we could establish an animal experimental model. Mice infected by ~10 CFU of bacteria were not active, decreased their weights dramatically and eventually died. Further analysis using mutant strains lacking in their virulence genes revealed that causative factor of mice death is Stx2f produced by the bacteria. Atrophied and desquamated epithelium was observed in the tubules of kidneys of the mice, similar to those of mice infected by enterohemorrhagic Escherichia coli. This animal model could be utilized to clarify the in-depth mechanisms of infection and pathogenicity of Stx2f-producing E. albertii.
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