Absorbance measurements for diagnosing alkaptonuria

• Project/Area Number: 16K19200
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Ehime Prefectural University of Health Science
• Principal Investigator: TOKUHARA YASUNORI 愛媛県立医療技術大学, 保健科学部, 講師(移行) (60746329)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
• Keywords: アルカプトン尿 / ホモゲンチジン酸 / ベンゾキノン酢酸 / 分光法 / 酸化剤 / 結晶次亜塩素酸ソーダ / アルカプトン尿症 / 吸光度

Outline of Final Research Achievements

Alkaptonuria is an amino acid metabolic disorder. The urine of patients with alkaptonuria turns dark brown at room temperature after several hours to days and this color change is accelerated by addition of alkali. In order to develop a facile
screening test for alkaptonuria, we added alkali to urine samples obtained from patients with alkaptonuria and measured the absorbance spectra. Addition of alkali to alkaptonuric urine exhibited characteristic absorption peaks at 406 nm and 430 nm. In addition, we added alkali with sodium hypochlorite pentahydrate, which is a strong oxidant, to alkaptonuric urine and observed changes in its color. Urine became a darker brown following the addition of alkali with sodium hypochlorite pentahydrate than with addition of alkali.

Research Products

• [Journal Article] Absorbance measurements of oxidation of homogentisic acid accelerated by the addition of alkaline solution with sodium hypochlorite pentahydrate (2018)
  • Author(s): Yasunori Tokuhara, Kenichi Shukuya, Masami Tanaka, Keisuke Sogabe, Yasukazu Ejima, Sho Hosokawa, Hiroyuki Ohsaki, Tatsuya Morinishi, Eiichiro Hirakawa, Yutaka Yatomi, Tatsuo Shimosawa
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 11364-11364
  • DOI: 10.1038/s41598-018-29769-w
  • NAID: 120006496805
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 酸化剤を用いたアルカプトン尿の暗褐色化促進の検討 (2017)
  • Author(s): 鈴木 七海，内藤 楓，仲宗根 由華，田中 雅美，宿谷 賢一，徳原 康哲．
  • Organizer: 第12回日本臨床検査学教育学会学術集会
• [Presentation] 酸化剤を用いたアルカプトン尿の暗褐色化促進の検討 (2017)
  • Author(s): 鈴木 七海, 内藤 楓, 仲宗根 由華, 田中 雅美, 宿谷 賢一, 徳原 康哲
  • Organizer: 第12回日本臨床検査学教育学会学術集会