| Project/Area Number |
16K19302
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
Taki Takashi 東京女子医科大学, 医学部, 講師 (70408475)
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| Research Collaborator |
KIBAYASHI Kazuhiko
MACHIDA Mitsuyo
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 体液識別 / 唾液 / miRNA / エクソソーム |
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| Outline of Final Research Achievements |
Although saliva is often left at crime scenes, no saliva-specific miRNAs have been reported so far. We hypothesized that salivary exosomes contain miRNAs that can be used as saliva-specific markers. In this study, we first prepared the pooled miRNAs derived from either saliva or salivary exosomes of eight donors. We then investigated the expression levels of 1088 miRNAs. We found that 85 miRNAs exhibited more than ten-fold differences in expression levels between saliva and salivary exosomes. We selected the miRNAs that exhibited the greatest differences in expression levels between saliva and salivary exosomes and examined their expression levels in the 8 individual sample. We found these miRNAs showed great variation in expression levels among the donors.
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