Epigenetic dysregulation of noncoding RNAs in gastrointestinal cancer progression
| Project/Area Number |
16K19352
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Research Collaborator |
Suzuki Hiromu 札幌医科大学, 分子生物学講座, 教授 (20381254)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | noncoding RNA / colon cancer / 大腸癌 / lncRNA / 大腸がん / 消化管がん / 転移 / エピジェネティクス / noncodingRNA |
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| Outline of Final Research Achievements |
First, we screened for noncoding RNAs which contribute to metastasis of gastrointestinal cancer from The Cancer Genome Atlas (TCGA) database. We selected COADREAD exon expression dataset, and found 21 lncRNA genes were upregulated in the group with lymph node metastasis compared to the group without metastasis. We assessed expression levels of these lncRNA genes in the series of colon cancer cell lines and normal colon tissues. In this analysis, DUXAP8 and DUXAP10 genes showed higher expression in cancer cells than normal tissues. Next, we examined DUXAP10 functions in colon cancer cells by knockdown using lentivirus vector. Depletion of DUXAP10 inhibited colon cancer cell growth and migration ability, thereby, DUXAP10 gene has oncogenic roles in colorectal cancer.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Epigenetic silencing of SMOC1 in traditional serrated adenoma and colorectal cancer.2017
Author(s)
Aoki H, Yamamoto E, Takasawa A, Niinuma T, Yamano HO, Harada T, Matsushita HO, Yoshikawa K, Takagi R, Harada E, Tanaka Y, Yoshida Y, Aoyama T, Eizuka M, Yorozu A, Kitajima H, Kai M, Sawada N, Sugai T, Nakase H, Suzuki H.
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Journal Title
Oncotarget.
Volume: 9
Issue: 4
Pages: 4707-4721
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Downregulation of miR-186 is associated with metastatic recurrence of gastrointestinal stromal tumors.2017
Author(s)
Niinuma T, Kai M, Kitajima H, Yamamoto E, Harada T, Maruyama R, Nobuoka T, Kanda T, Hasegawa T, Tokino T, Sugai T, Shimomura Y, Nakase H, Suzuki H
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Journal Title
Oncology Letters
Volume: 14
Pages: 5703-5710
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer.2017
Author(s)
Kai M, Yamamoto E, Sato A, Yamano HO, Niinuma T, Kitajima H, Harada T, Aoki H, Maruyama R, Toyota M, Hatahira T, Nakase H, Sugai T, Yamashita T, Toyota M, Suzuki H.
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Journal Title
Mol Carcinog
Volume: -
Issue: 7
Pages: 1743-1752
DOI
Related Report
Peer Reviewed / Open Access
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