| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
A knockdown of perlecan, a basement membrane component, in Human Aortic Endothelial Cells (HAEC) by perlecan siRNA caused a reduced expression of endothelial NO synthase (eNOS) but not a reduced phosphorylated eNOS expression. A Src kinase phosphorylation, Akt phosphorylation and VEGF receptor 2 expressions involved in the regulation of eNOS expression and eNOS phosphorylation were decreased by knockdown of perlecan in HAEC. These results indicated that perlecan related eNOS expressions via Src/Akt signaling pathway. Analysis of perlecan functional fragments indicates that perlecan functional domain V is less involved in the regulation of eNOS expression.
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